Surachat Komwit, Narkthewan Patcharaporn, Thotsagotphairee Chayanin, Wonglapsuwan Monwadee, Thongpradub Walaiporn
Department of Biomedical Science and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Molecular Evolution and Computational Biology Research Unit, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Animals (Basel). 2022 Jul 10;12(14):1769. doi: 10.3390/ani12141769.
(short mackerel) and (Indian mackerel) are commercially important marine species in Southeast Asia. In recent years, numbers of these two species have been decreasing in the wild, and genomic information about them is still limited. We conducted a genome survey of these two mackerel species to acquire essential genomic information using next-generation sequencing data. To obtain this genetic information, comprehensive bioinformatics analyses were performed, including de novo assembly, gene prediction, functional annotation, and phylogenetic analysis. The estimated genome sizes were around 680.14 Mbp () and 688.82 Mbp (). The heterozygosity of these species was very similar (≈0.81), while the repeat content for (9.30%) was slightly higher than for (8.30%). Functional annotation indicated that most of the genes predicted in these two species shared very close average amino acid identities (94.06%). The phylogenetic analysis revealed close phylogenetic relationships between these two species and other scombrids. This is the first reported genome survey and assembly of species in the genus and could be useful for future comparative genomic studies.
(短鲭)和(印度鲭)是东南亚具有重要商业价值的海洋物种。近年来,这两个物种在野外的数量一直在减少,而且关于它们的基因组信息仍然有限。我们利用下一代测序数据对这两种鲭鱼进行了基因组调查,以获取基本的基因组信息。为了获得这些遗传信息,我们进行了全面的生物信息学分析,包括从头组装、基因预测、功能注释和系统发育分析。估计的基因组大小分别约为680.14兆碱基对()和688.82兆碱基对()。这些物种的杂合度非常相似(约为0.81),而(9.30%)的重复序列含量略高于(8.30%)。功能注释表明,在这两个物种中预测的大多数基因具有非常接近的平均氨基酸同一性(94.06%)。系统发育分析揭示了这两个物种与其他鲭科鱼类之间密切的系统发育关系。这是首次报道的属物种的基因组调查和组装,可能对未来的比较基因组研究有用。