Kyono ART Clinic, Mitsui-Seimei, Sendai Honcho Bl, 3F, 1-1-1, Honcho, Aobaku, Sendai, 980-0014, Japan.
J Assist Reprod Genet. 2009 Aug;26(8):451-3. doi: 10.1007/s10815-009-9338-2. Epub 2009 Sep 17.
To report a successful delivery of a healthy baby after transfer of vitrified-warmed blastocysts derived from introcytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm.
A female patient and her husband with non-obstructive azoospermia received a transfer of vitrified-warmed blastocysts from vitrified-warmed oocytes and frozen-thawed sperm. The main outcome measures were fertilization, pregnancy and birth.
Nine oocytes were matured and vitrified. When the vitrified oocytes were warmed, six survived with good quality morphology. Using ICSI, frozen-thawed sperm was injected into the six warmed oocytes that survived, and the fertilization rate was 100%. The zygotes were cultured, and five of six early embryos became blastocysts. One of them was transferred, but pregnancy was not achieved. The second time around, two vitrified-warmed blastocysts were transferred resulting in pregnancy, and a healthy boy was delivered.
This is a rare case of a successful birth using a vitrified-warmed blastocyst grown after ICSI with a vitrified-warmed oocyte and frozen-thawed sperm.
报告一例通过玻璃化冷冻-解冻卵母细胞和冻融精子的卵胞浆内单精子注射（ICSI）衍生的玻璃化冷冻-解冻囊胚移植后成功分娩健康婴儿。
一名患有非梗阻性无精子症的女性患者和她的丈夫接受了玻璃化冷冻-解冻囊胚的移植，这些囊胚来自玻璃化冷冻-解冻卵母细胞和冻融精子。主要观察指标为受精、妊娠和分娩。
成熟并玻璃化了 9 个卵母细胞。当玻璃化的卵母细胞被解冻时，有 6 个存活且形态良好。使用 ICSI 将冻融精子注入 6 个存活的解冻卵母细胞中，受精率为 100%。将受精卵培养，其中 6 个早期胚胎中的 5 个发育成囊胚。其中一个进行了移植，但未妊娠。第二次移植了两个玻璃化冷冻-解冻囊胚，结果妊娠，并分娩了一个健康的男婴。
这是一例罕见的成功分娩病例，使用玻璃化冷冻-解冻卵母细胞和冻融精子的 ICSI 后培养的玻璃化冷冻-解冻囊胚成功妊娠并分娩。
