Compensatory Upregulation of LPA and Activation of the PI3K-Akt Pathway Prevent LPA-Dependent Loss of Intestinal Epithelial Cells in Intestinal Organoids.

Renewal of the intestinal epithelium is orchestrated by regenerative epithelial proliferation within crypts. Recent studies have shown that lysophosphatidic acid (LPA) can maintain intestinal epithelial renewal in vitro and conditional deletion of () in mice ablates the intestinal epithelium and increases morbidity. In contrast, constitutive deletion () does not cause a defect in intestinal crypt regeneration. In this study, we investigated whether another LPA receptor (LPAR) compensates for constitutive loss of LPA function to allow regeneration of intestinal epithelium. In intestinal epithelial cells (IECs), was upregulated and blocking LPA function reduced proliferation and increased apoptosis of IECs. Similar to mice, the absence of () resulted in upregulation of in IECs, indicating that LPA and LPA reciprocally compensate for the loss of each other. Blocking LPA in enteroids reduced phosphorylation of Akt, indicating that LPA maintains the growth of enteroids through activation of the PI3K-Akt pathway. The present study provides evidence that loss of an LPAR can be compensated by another LPAR. This ability to compensate needs to be considered in studies aimed to define receptor functions or test the efficacy of a LPAR-targeting drug using genetically engineered animal models.