Fernandes Liliana, Ribeiro Rita, Costa Raquel, Henriques Mariana, Rodrigues M Elisa
Centre of Biological Engineering, LMaS-Laboratório de Microbiologia Aplicada à Saúde, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal.
LABBELS-Associate Laboratory, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal.
Antibiotics (Basel). 2022 Jul 20;11(7):977. doi: 10.3390/antibiotics11070977.
is a recently found species, mainly associated with nosocomial outbreaks in intensive care hospital settings, and unlike other species, it can be transmitted through person-to-person or by contact with surfaces. is described as resistant to first-line antifungals and, consequently, associated with high mortality. Nowadays, essential oils (EOs) are known to be effective against fungal and bacterial infections. This work aimed to evaluate the effect of four EOs (tea tree, niaouli, white thyme and cajeput) against . The EO's effect on planktonic growth was evaluated by the minimum inhibitory concentration determination and by the agar disc diffusion method. Then, the same effect was evaluated on biofilm by colony-forming units' enumeration. The results showed that EOs were able to inhibit the planktonic growth, with an MIC50 between 0.78 and 1.56% and halos of 20-21 mm for white thyme and tea tree and 13-14 mm for cajeput and niaouli. In addition, the EOs were also able to completely inhibit biofilm formation. Moreover, white thyme and cajeput completely eradicate pre-formed biofilms, while tea tree and niaouli significantly reduce it. Thus, this work demonstrates that EOs are a possible therapeutic alternative and a future perspective for the hard fight against .
是一种最近发现的物种，主要与重症监护医院环境中的医院感染暴发有关，与其他物种不同，它可以通过人际传播或通过接触表面传播。被描述为对一线抗真菌药物耐药，因此与高死亡率相关。如今，已知精油（EOs）对真菌和细菌感染有效。这项工作旨在评估四种精油（茶树、绿花白千层、白百里香和玉树）对的作用。通过最小抑菌浓度测定和琼脂圆盘扩散法评估精油对浮游生长的作用。然后，通过菌落形成单位计数对生物膜的相同作用进行评估。结果表明，精油能够抑制浮游生长，白百里香和茶树的MIC50在0.78%至1.56%之间，抑菌圈为20 - 21毫米，玉树和绿花白千层的抑菌圈为13 - 14毫米。此外，精油还能够完全抑制生物膜的形成。此外，白百里香和玉树能完全消除预先形成的生物膜，而茶树和绿花白千层能显著减少生物膜。因此，这项工作表明，精油是对抗的艰苦斗争中一种可能的治疗选择和未来前景。
