Department of Chemistry, Hacettepe University, Ankara 06800, Turkey.
Department of Chemistry Technology, Bolu Abant Izzet Baysal University, Bolu 14900, Turkey.
Biosensors (Basel). 2022 Jul 1;12(7):482. doi: 10.3390/bios12070482.
Herein, gold nanoparticles (AuNP)-modified cortisol-imprinted (AuNP-MIP) plasmonic sensor was developed for signal amplification and real-time cortisol determination in both aqueous and complex solutions. Firstly, the sensor surfaces were modified with 3-(trimethoxylyl)propyl methacrylate and then pre-complex was prepared using the functional monomer N-methacryloyl-L-histidine methyl ester. The monomer solution was made ready for polymerization by adding 2-hydroxyethyl methacrylate to ethylene glycol dimethacrylate. In order to confirm the signal enhancing effect of AuNP, only cortisol-imprinted (MIP) plasmonic sensor was prepared without AuNP. To determine the selectivity efficiency of the imprinting process, the non-imprinted (AuNP-NIP) plasmonic sensor was also prepared without cortisol. The characterization studies of the sensors were performed with atomic force microscopy and contact angle measurements. The kinetic analysis of the AuNP-MIP plasmonic sensor exhibited a high correlation coefficient (R = 0.97) for a wide range (0.01-100 ppb) with a low detection limit (0.0087 ppb) for cortisol detection. Moreover, the high imprinting efficiency (k' = 9.67) of the AuNP-MIP plasmonic sensor was determined by comparison with the AuNP-NIP plasmonic sensor. All kinetic results were validated and confirmed by HPLC.
本文开发了一种金纳米粒子（AuNP）修饰的皮质醇印迹（AuNP-MIP）等离子体传感器，用于在水相和复杂溶液中进行信号放大和实时皮质醇测定。首先，用 3-（三甲氧基甲硅烷基）丙基甲基丙烯酸酯修饰传感器表面，然后用功能单体 N-丙烯酰基-L-组氨酸甲酯制备预复合物。将甲基丙烯酸羟乙酯加入到乙二醇二甲基丙烯酸酯中，使单体溶液为聚合做好准备。为了确认 AuNP 的信号增强效果，仅制备了没有 AuNP 的皮质醇印迹（MIP）等离子体传感器。为了确定印迹过程的选择性效率，还制备了没有皮质醇的非印迹（AuNP-NIP）等离子体传感器。传感器的特征研究是通过原子力显微镜和接触角测量进行的。AuNP-MIP 等离子体传感器的动力学分析显示，对于宽范围（0.01-100 ppb），具有高相关系数（R = 0.97），皮质醇检测的检测限低（0.0087 ppb）。此外，通过与 AuNP-NIP 等离子体传感器进行比较，确定了 AuNP-MIP 等离子体传感器的高印迹效率（k'=9.67）。所有动力学结果均通过 HPLC 进行验证和确认。
