Abdulsattar Jwan O, Greenway Gillian M, Wadhawan Jay D
Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq.
Department of Chemistry and Biochemistry, University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Heliyon. 2020 Mar 18;6(3):e03558. doi: 10.1016/j.heliyon.2020.e03558. eCollection 2020 Mar.
A rapid electrochemical immunoassay method was developed to detect and measure stress biomarkers (cortisol and cortisone) in two biological samples (Zebrafish whole-body and artificial saliva). This methodology utilizes an immunoassay approach taking advantage of the lock and key mechanism that is related to the antibody-antigen interaction depending on the reliable immobilization of the antibody labelled with ferrocene tags (Ab-Fc) on a modified tin-doped indium oxide (ITO) electrode using electrochemical instrumentation to build a POC platform. The limit of detection (LOD) obtained for this biosensor was 1.03 pg ml for cortisol and 0.68 pg ml for cortisone, respectively. The correlation coefficient was 0.9852 and 0.9841 for cortisol and cortisone, respectively with a linear concentration from (0-50 ng ml) which covers the standard levels of stress hormones in both selected biological samples. The incubation time was investigated and 30 min was found to be the optimum incubation time. This time would be acceptable for the POC system as total process time can be determined within 35 min.
开发了一种快速电化学免疫分析方法，用于检测和测量两种生物样品（斑马鱼全身和人工唾液）中的应激生物标志物（皮质醇和可的松）。该方法采用免疫分析方法，利用与抗体 - 抗原相互作用相关的锁钥机制，通过电化学仪器将标记有二茂铁标签的抗体（Ab-Fc）可靠地固定在修饰的掺锡氧化铟（ITO）电极上，构建一个即时检测（POC）平台。该生物传感器对皮质醇的检测限（LOD）为1.03 pg/ml，对可的松的检测限为0.68 pg/ml。皮质醇和可的松的相关系数分别为0.9852和0.9841，线性浓度范围为（0 - 50 ng/ml），涵盖了两种选定生物样品中应激激素的标准水平。研究了孵育时间，发现30分钟是最佳孵育时间。由于整个过程时间可在35分钟内确定，这个时间对于POC系统来说是可以接受的。
