Takaya Kento, Sunohara Ayano, Aramaki-Hattori Noriko, Sakai Shigeki, Okabe Keisuke, Kanazawa Hideko, Asou Toru, Kishi Kazuo
Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
Biomedicines. 2022 Jun 28;10(7):1536. doi: 10.3390/biomedicines10071536.
Wnt proteins secrete glycoproteins that are involved in various cellular processes to maintain homeostasis during development and adulthood. However, the expression and role of Wnt in wound healing have not been fully documented. Our previous studies have shown that, in an early-stage mouse fetus, no scarring occurred after cutaneous wounding, and complete regeneration was achieved. In this study, the expression and localization of Wnt proteins in a mouse fetal-wound-healing model and their associations with scar formation were analyzed. Wnt-related molecules were detected by in-situ hybridization, immunostaining, and real-time polymerase chain reaction. The results showed altered expression of Wnt-related molecules during the wound-healing process. Moreover, scar formation was suppressed by Wnt inhibitors, suggesting that Wnt signaling may be involved in wound healing and scar formation. Thus, regulation of Wnt signaling may be a possible mechanism to control scar formation.
Wnt蛋白分泌糖蛋白，这些糖蛋白参与各种细胞过程，以在发育和成年期维持体内平衡。然而，Wnt在伤口愈合中的表达和作用尚未得到充分记录。我们之前的研究表明，在早期小鼠胎儿中，皮肤受伤后不会形成瘢痕，而是实现完全再生。在本研究中，分析了Wnt蛋白在小鼠胎儿伤口愈合模型中的表达和定位及其与瘢痕形成的关联。通过原位杂交、免疫染色和实时聚合酶链反应检测Wnt相关分子。结果显示，在伤口愈合过程中Wnt相关分子的表达发生了改变。此外，Wnt抑制剂可抑制瘢痕形成，这表明Wnt信号可能参与伤口愈合和瘢痕形成。因此，调节Wnt信号可能是控制瘢痕形成的一种可能机制。
