Madyarova Ekaterina, Shirokova Yulia, Gurkov Anton, Drozdova Polina, Baduev Boris, Lubyaga Yulia, Shatilina Zhanna, Vishnevskaya Maria, Timofeyev Maxim
Institute of Biology, Irkutsk State University, 664025 Irkutsk, Russia.
Baikal Research Centre, 664011 Irkutsk, Russia.
Insects. 2022 Jun 24;13(7):578. doi: 10.3390/insects13070578.
Lake Baikal is the only freshwater reservoir inhabited by deep-water fauna, which originated mostly from shallow-water ancestors. and are endemic scavenger amphipods (Amphipoda, Crustacea) dwelling in wide depth ranges of the lake covering over 1300 m. had been previously collected close to the surface, while has never been found above the depth of 47 m. Since is a promising model species for various research, here we tested whether is less metabolically adapted to atmospheric pressure than . We analyzed a number of energy-related traits (contents of glucose, glycogen and adenylates, as well as lactate dehydrogenase activity) and oxidative stress markers (activities of antioxidant enzymes and levels of lipid peroxidation products) after sampling from different depths and after both species' acclimation to atmospheric pressure. The analyses were repeated in two independent sampling campaigns. We found no consistent signs of metabolic disturbances or oxidative stress in both species right after lifting. Despite surviving slightly better in laboratory conditions, during long-term acclimation, both species showed comparable reactions without critical changes. Thus, the obtained data favor using along with for physiological research under laboratory conditions.
贝加尔湖是唯一栖息着深水动物群的淡水水库，这些动物群大多起源于浅水祖先。 是特有的食腐性端足类动物（端足目，甲壳纲），生活在该湖超过1300米的广泛深度范围内。 此前曾在靠近水面处采集到，而 在47米深度以上从未被发现过。由于 是各种研究中有前景的模式物种，在这里我们测试了 在代谢上是否比 更不适应大气压力。我们在从不同深度采样后以及两种物种适应大气压力后，分析了一些与能量相关的特征（葡萄糖、糖原和腺苷酸的含量，以及乳酸脱氢酶活性）和氧化应激标志物（抗氧化酶的活性和脂质过氧化产物的水平）。这些分析在两次独立的采样活动中重复进行。我们发现两种物种在被捞出后，都没有一致的代谢紊乱或氧化应激迹象。尽管 在实验室条件下存活得稍好一些，但在长期适应过程中，两种物种都表现出类似的反应，没有关键变化。因此，获得的数据支持在实验室条件下将 与 一起用于生理学研究。
