• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

乙烯应答因子 ERF5 通过与 和 基因互作调控‘紫金’桑椹果实花色素苷合成。

The Ethylene Response Factor ERF5 Regulates Anthocyanin Biosynthesis in 'Zijin' Mulberry Fruits by Interacting with and Genes.

机构信息

Academy of Agricultural Sciences, Industrial Crops Institute of Hubei, Wuhan 430064, China.

National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

出版信息

Int J Mol Sci. 2022 Jul 9;23(14):7615. doi: 10.3390/ijms23147615.

DOI:10.3390/ijms23147615
PMID:35886963
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9318412/
Abstract

Ethylene promotes ripening in fruits as well as the biosynthesis of anthocyanins in plants. However, the question of which ethylene response factors (ERFs) interact with the genes along the anthocyanin biosynthesis pathway is yet to be answered. Herein, we conduct an integrated analysis of transcriptomes and metabolome on fruits of two mulberry genotypes ('Zijin', ZJ, and 'Dashi', DS, with high and low anthocyanin abundance, respectively) at different post-flowering stages. In total, 1035 upregulated genes were identified in ZJ and DS, including in the MBW complex and anthocyanin related genes such as . A KEGG analysis suggested that flavonoid biosynthesis and plant hormone signaling transduction pathways were significantly enriched in the upregulated gene list. In particular, among 103 genes, the expression of showed the most positive correlation with the anthocyanin change pattern across both genotypes and in the post-flowering stages, with a Pearson correlation coefficient (PCC) of 0.93. Electrophoresis mobility shift assay (EMSA) and luciferase assay suggested that ERF5 binds to the promoter regions of MYBA and F3H and transcriptionally activates their gene expression. We elucidated a potential mechanism by which ethylene enhances anthocyanin accumulation in mulberry fruits and highlighted the importance of the gene in controlling the anthocyanin content in mulberry species. This knowledge could be used for engineering purposes in future mulberry breeding programs.

摘要

乙烯促进果实成熟和植物中花色苷的生物合成。然而，目前尚不清楚哪些乙烯反应因子（ERFs）与花色苷生物合成途径中的基因相互作用。在这里，我们对两个桑树种（‘Zijin’，ZJ 和‘Dashi’，DS，花色苷含量高和低）在不同开花后阶段的果实进行了转录组和代谢组的综合分析。总共在 ZJ 和 DS 中鉴定到 1035 个上调基因，包括 MBW 复合物和花色苷相关基因，如 。KEGG 分析表明，黄酮类生物合成和植物激素信号转导途径在上调基因列表中显著富集。特别是在 103 个基因中，基因的表达与两个基因型和开花后阶段的花色苷变化模式呈最正相关，皮尔逊相关系数（PCC）为 0.93。电泳迁移率变动分析（EMSA）和荧光素酶测定表明，ERF5 结合到 MYBA 和 F3H 的启动子区域，并转录激活它们的基因表达。我们阐明了乙烯增强桑树果实中花色苷积累的潜在机制，并强调了 基因在控制桑树物种花色苷含量中的重要性。这一知识可用于未来桑树育种计划中的工程设计。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/fb6e18216f3c/ijms-23-07615-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/dc1c813f429a/ijms-23-07615-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/fa69beca61fb/ijms-23-07615-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/c679b18a6878/ijms-23-07615-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/2974208aafe5/ijms-23-07615-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/514c94f9374c/ijms-23-07615-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/38db50bc1dc7/ijms-23-07615-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/a8c6cf7acd57/ijms-23-07615-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/fb6e18216f3c/ijms-23-07615-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/dc1c813f429a/ijms-23-07615-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/fa69beca61fb/ijms-23-07615-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/c679b18a6878/ijms-23-07615-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/2974208aafe5/ijms-23-07615-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/514c94f9374c/ijms-23-07615-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/38db50bc1dc7/ijms-23-07615-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/a8c6cf7acd57/ijms-23-07615-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe34/9318412/fb6e18216f3c/ijms-23-07615-g008.jpg

相似文献

1
The Ethylene Response Factor ERF5 Regulates Anthocyanin Biosynthesis in 'Zijin' Mulberry Fruits by Interacting with and Genes.乙烯应答因子 ERF5 通过与 和 基因互作调控‘紫金’桑椹果实花色素苷合成。
Int J Mol Sci. 2022 Jul 9;23(14):7615. doi: 10.3390/ijms23147615.
2
Functional Characterization of Flavanone 3-Hydroxylase (F3H) and Its Role in Anthocyanin and Flavonoid Biosynthesis in Mulberry.解析黄酮醇 3-羟化酶（F3H）的功能及其在桑树中花色苷和类黄酮生物合成中的作用。
Molecules. 2022 May 23;27(10):3341. doi: 10.3390/molecules27103341.
3
Transcriptomic and metabolomic analyses reveal the importance of ethylene networks in mulberry fruit ripening.转录组学和代谢组学分析揭示了乙烯网络在桑椹果实成熟过程中的重要性。
Plant Sci. 2024 Jul;344:112084. doi: 10.1016/j.plantsci.2024.112084. Epub 2024 Apr 12.
4
Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in 'Red Zaosu' pear fruits by interacting with MYB114.乙烯反应因子 Pp4ERF24 和 Pp12ERF96 通过与 MYB114 互作调控‘红早酥’梨果实中蓝光诱导的花青苷合成。
Plant Mol Biol. 2019 Jan;99(1-2):67-78. doi: 10.1007/s11103-018-0802-1. Epub 2018 Dec 11.
5
Cloning and expression analyses of the anthocyanin biosynthetic genes in mulberry plants.桑树中花青素生物合成基因的克隆与表达分析
Mol Genet Genomics. 2014 Oct;289(5):783-93. doi: 10.1007/s00438-014-0851-3. Epub 2014 Apr 21.
6
Comparative Transcriptomic Profiling to Understand Pre- and Post-Ripening Hormonal Regulations and Anthocyanin Biosynthesis in Early Ripening Apple Fruit.比较转录组学分析揭示早采苹果果实成熟过程中激素调控和花色苷生物合成的机制
Molecules. 2018 Jul 31;23(8):1908. doi: 10.3390/molecules23081908.
7
Ethylene Inhibits Anthocyanin Biosynthesis by Repressing the R2R3-MYB Regulator in Tomato.乙烯通过抑制番茄中的 R2R3-MYB 调控因子抑制花色素苷生物合成。
Int J Mol Sci. 2022 Jul 11;23(14):7648. doi: 10.3390/ijms23147648.
8
Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in 'Zaosu' Pear ( Rehd.) Fruit.茉莉酸和乙烯调控的乙烯响应因子 22 促进‘早酥’梨果实中羊毛脂诱导的花色苷生物合成。
Biomolecules. 2020 Feb 11;10(2):278. doi: 10.3390/biom10020278.
9
Molecular characterization of anthocyanin and betulinic acid biosynthesis in red and white mulberry fruits using high-throughput sequencing.利用高通量测序对红白桑椹果实中花色苷和白桦脂酸生物合成的分子特征进行分析。
Food Chem. 2019 May 1;279:364-372. doi: 10.1016/j.foodchem.2018.11.101. Epub 2018 Nov 29.
10
Ethylene-activated PpERF105 induces the expression of the repressor-type R2R3-MYB gene PpMYB140 to inhibit anthocyanin biosynthesis in red pear fruit.乙烯激活 PpERF105 诱导抑制型 R2R3-MYB 基因 PpMYB140 的表达，从而抑制红皮梨果实中花色苷的生物合成。
Plant J. 2021 Jan;105(1):167-181. doi: 10.1111/tpj.15049. Epub 2020 Nov 21.

引用本文的文献

1
Functional analysis of AP2/ERF family members in flavonoid biosynthesis of wolfberry.枸杞黄酮生物合成中AP2/ERF家族成员的功能分析
Front Plant Sci. 2025 Sep 2;16:1632482. doi: 10.3389/fpls.2025.1632482. eCollection 2025.
2
Comparative metabolomics reveal the biochemical basis of mulberry fruit decay during low temperature storage.比较代谢组学揭示了低温贮藏期间桑椹果实腐烂的生化基础。
Food Chem X. 2025 May 23;28:102568. doi: 10.1016/j.fochx.2025.102568. eCollection 2025 May.
3
Ethylene modulates the phenylpropanoid pathway by enhancing expression via the ERF5-melatonin-ERF104 pathway in grape seeds.

本文引用的文献

1
Functional characterization of two chalcone isomerase (CHI) revealing their responsibility for anthocyanins accumulation in mulberry.两种查尔酮异构酶（CHI）的功能特征分析揭示了它们在桑椹花青苷积累中的作用。
Plant Physiol Biochem. 2021 Apr;161:65-73. doi: 10.1016/j.plaphy.2021.01.044. Epub 2021 Feb 3.
2
Comparative transcriptome analysis of mulberry reveals anthocyanin biosynthesis mechanisms in black (Morus atropurpurea Roxb.) and white (Morus alba L.) fruit genotypes.桑树种质资源比较转录组分析揭示了黑色（MorusalbaL.）和白色（MorusatropurpureaRoxb.）桑椹果实花色苷生物合成的机制。
BMC Plant Biol. 2020 Jun 17;20(1):279. doi: 10.1186/s12870-020-02486-1.
3
乙烯通过增强葡萄种子中经由ERF5-褪黑素-ERF104途径的表达来调节苯丙烷类代谢途径。
Hortic Res. 2025 Feb 25;12(6):uhaf061. doi: 10.1093/hr/uhaf061. eCollection 2025 Jun.
4
Transcription Factor Positively Regulates Anthocyanin Accumulation in Grape ().转录因子正向调控葡萄中的花青素积累（）。
Plants (Basel). 2025 Mar 11;14(6):871. doi: 10.3390/plants14060871.
5
Transcriptome analyses for revealing leaf abscission of Cyclocarya paliurus stem segments in vitro.转录组分析揭示青钱柳茎段离体叶片脱落情况
BMC Genomics. 2025 Mar 3;26(1):208. doi: 10.1186/s12864-025-11394-3.
6
Expression of ABC transporters negatively correlates with ectoine biosynthesis in Halomonas campaniensis under NaCl and ultraviolet mutagenesis treatments revealed by transcriptomic and proteomics combined analysis.通过转录组学和蛋白质组学联合分析揭示，在 NaCl 和紫外线诱变处理下，ABC 转运蛋白的表达与嗜盐菌中海盐单胞菌中海盐嘧啶核苷的生物合成呈负相关。
BMC Genomics. 2024 Nov 20;25(1):1114. doi: 10.1186/s12864-024-11003-9.
7
Responses of transcriptome and metabolome in peanut leaves to dibutyl phthalate during whole growth period.花生叶片转录组和代谢组在整个生长周期对邻苯二甲酸二丁酯的响应
Front Plant Sci. 2024 Sep 20;15:1448971. doi: 10.3389/fpls.2024.1448971. eCollection 2024.
8
Transcriptomic Analysis Reveals the Flavonoid Biosynthesis Pathway Involved in Rhizome Development in Hua.转录组分析揭示了参与华重楼根茎发育的类黄酮生物合成途径。
Plants (Basel). 2024 May 31;13(11):1524. doi: 10.3390/plants13111524.
9
Functional Characterization of F3H Gene and Optimization of Dihydrokaempferol Biosynthesis in .F3H 基因功能表征及二氢山柰酚生物合成的优化
Molecules. 2024 May 8;29(10):2196. doi: 10.3390/molecules29102196.
10
For a Colorful Life: Recent Advances in Anthocyanin Biosynthesis during Leaf Senescence.为了多彩的生活：叶片衰老过程中花青素生物合成的最新进展
Biology (Basel). 2024 May 9;13(5):329. doi: 10.3390/biology13050329.
Overexpression of maize transcription factor mEmBP-1 increases photosynthesis, biomass, and yield in rice.
玉米转录因子mEmBP-1的过表达提高了水稻的光合作用、生物量和产量。
J Exp Bot. 2020 Aug 6;71(16):4944-4957. doi: 10.1093/jxb/eraa248.
4
Combined Proteomics and Metabolism Analysis Unravels Prominent Roles of Antioxidant System in the Prevention of Alfalfa ( L.) against Salt Stress.联合蛋白质组学和代谢分析揭示抗氧化系统在苜蓿（L.）抵御盐胁迫中的重要作用。
Int J Mol Sci. 2020 Jan 30;21(3):909. doi: 10.3390/ijms21030909.
5
Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different Varieties.不同品种富含多酚的块茎的植物化学特征比较、遗传特征分析及抗增殖活性研究
Molecules. 2020 Jan 6;25(1):233. doi: 10.3390/molecules25010233.
6
The ERF transcription factor MdERF38 promotes drought stress-induced anthocyanin biosynthesis in apple.ERF 转录因子 MdERF38 促进苹果干旱胁迫诱导的花青素生物合成。
Plant J. 2020 Feb;101(3):573-589. doi: 10.1111/tpj.14555. Epub 2019 Nov 10.
7
The function of genes in the light-induced anthocyanin production of leaves.基因在叶片光诱导花青素生成中的作用。
Plant Biotechnol (Tokyo). 2018;35(1):87-91. doi: 10.5511/plantbiotechnology.18.0122b. Epub 2018 Mar 10.
8
AP2/ERF Transcription Factor Regulatory Networks in Hormone and Abiotic Stress Responses in .植物中激素和非生物胁迫响应中的AP2/ERF转录因子调控网络
Front Plant Sci. 2019 Feb 28;10:228. doi: 10.3389/fpls.2019.00228. eCollection 2019.
9
SlMYB75, an MYB-type transcription factor, promotes anthocyanin accumulation and enhances volatile aroma production in tomato fruits.SlMYB75是一种MYB型转录因子，可促进番茄果实中花青素的积累并增强挥发性香气的产生。
Hortic Res. 2019 Feb 1;6:22. doi: 10.1038/s41438-018-0098-y. eCollection 2019.
10
Completion of the cytosolic post-chorismate phenylalanine biosynthetic pathway in plants.植物细胞质中支链氨基酸生物合成途径的完成。
Nat Commun. 2019 Jan 3;10(1):15. doi: 10.1038/s41467-018-07969-2.