Park Kyung Hee, Song Ho-Jun, Park Yeong-Joon
Department of Dental Materials and Hard-Tissue Biointerface Research Center, School of Dentistry, Chonnam National University, Gwangju 61186, Korea.
Materials (Basel). 2022 Jul 7;15(14):4773. doi: 10.3390/ma15144773.
Calcium phosphate has attracted enormous attention as a bone regenerative material in biomedical fields. In this study, we investigated the effect of microwave treatment on calcium phosphate deposited TiO nanoflower to improve protein adsorption. Hierarchical rutile TiO nanoflowers (TiNF) fabricated by a hydrothermal method were soaked in modified simulated body fluid for 3 days to induce calcium phosphate (CAP) formation, followed by exposure to microwave radiation (MW). Coating the dental implants with CAP/TiNF provides a means of improving the biological properties, as the structure, morphology, and thickness of the composites can be controlled. The composites were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), field emission transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR), respectively. The composites were identified to be composed of aggregated nano-sized particles with sphere-like shapes, and the calcium phosphate demonstrated low crystallinity. The ability of bovine serum albumin (BSA) to adsorb on MW-treated CAP/TiNF composites was studied as a function of BSA concentration. The Sips isotherm was used to analyze the BSA adsorption on MW-treated CAP/TiNF composites. The MW-treated samples showed high protein adsorption capacity, thereby indicating their potential in various biomedical applications.
磷酸钙作为生物医学领域的骨再生材料受到了广泛关注。在本研究中，我们研究了微波处理对沉积有磷酸钙的TiO纳米花的影响，以改善蛋白质吸附。通过水热法制备的分级金红石TiO纳米花（TiNF）浸泡在改性模拟体液中3天以诱导磷酸钙（CAP）形成，随后暴露于微波辐射（MW）。用CAP/TiNF涂覆牙科植入物提供了一种改善生物学性能的方法，因为复合材料的结构、形态和厚度可以得到控制。分别通过扫描电子显微镜（SEM）、X射线衍射（XRD）、场发射透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）对复合材料进行表征。复合材料被鉴定为由聚集的球形纳米颗粒组成，并且磷酸钙显示出低结晶度。研究了牛血清白蛋白（BSA）在微波处理的CAP/TiNF复合材料上的吸附能力与BSA浓度的关系。采用Sips等温线分析BSA在微波处理的CAP/TiNF复合材料上的吸附。微波处理的样品显示出高蛋白质吸附能力，从而表明它们在各种生物医学应用中的潜力。
