Posgrado en Ciencia e Ingeniería de Materiales, Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Campus Juriquilla, 76230, Querétaro, Qro., Mexico.
Licenciatura en Tecnología, Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Campus Juriquilla, 76230, Querétaro, Qro., Mexico.
Sci Rep. 2019 Apr 11;9(1):5915. doi: 10.1038/s41598-019-42269-9.
This paper focuses on the study of the effect of the change of the crystal size on the shape and width of the X-ray diffraction patterns for defatted and deproteinized bones as well as incinerated biogenic hydroxyapatite obtained from bovine, porcine, and human bones. Inductively Couple Plasma showed the presence of some ions such as Mg, K, Al, Fe, Zn, and Na for all samples. The nanometric size of the crystals was determined through High Resolution Transmission Electron Microscopy in which ordered crystals were found. The calcination of raw clean bones at 720 °C produced a transition of crystal size from nano to micro due to a coalescence phenomenon, this was accompanied by a decrease of the peak width of the X-ray diffraction patterns due to the decrease of the inelastic scattering contribution from the microcrystals. A simulation of the effect of the crystallite size on the shape and width of the X-ray patterns was done using PDF-4 software which confirmed that raw ordered bone crystals produce broad peaks which so far have been erroneously assigned to polycrystalline hydroxyapatite with low crystalline quality.
本文专注于研究脱脂脱蛋白骨以及从牛、猪和人骨中获得的燃烧生物羟基磷灰石的晶体尺寸变化对 X 射线衍射图谱的形状和宽度的影响。感应耦合等离子体显示所有样品中都存在一些离子，如 Mg、K、Al、Fe、Zn 和 Na。通过高分辨率透射电子显微镜确定了纳米级大小的晶体，其中发现了有序晶体。在 720°C 下煅烧原始清洁骨会由于聚结现象导致晶体尺寸从纳米到微米的转变，这伴随着 X 射线衍射图谱的峰宽减小，这是由于微晶的非弹性散射贡献减小所致。使用 PDF-4 软件模拟了晶粒尺寸对 X 射线图谱形状和宽度的影响，该模拟证实，原始有序骨晶体产生宽峰，迄今为止，这些宽峰一直被错误地分配给结晶质量低的多晶羟基磷灰石。
