Zhang Xiaoxu, Liu Xiaoxue, Yang Jiufang, Ren Fazheng, Li Yixuan
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Metabolites. 2022 Jul 11;12(7):633. doi: 10.3390/metabo12070633.
A simple, sensitive, and reliable quantification and identification method was developed and validated for simultaneous analysis of 58 bile acids (BAs) in human and rodent (mouse and rat) fecal samples. The method involves an extraction step with a 5% ammonium-ethanol aqueous solution; the BAs were quantified by high-resolution mass spectrometry (ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry, UPLC-Q-TOF). The recoveries were 80.05-120.83%, with coefficient variations (CVs) of 0.01-9.82% for three biological species. The limits of detection (LODs) were in the range of 0.01-0.24 μg/kg, and the limits of quantification (LOQs) ranged from 0.03 to 0.81 μg/kg. In addition, the analytical method was used to identify and quantify BAs in end-stage renal disease (ESRD) patients, C57BL/6 mice, and Sprague-Dawley (SD) rats. The fecal BA profile and analysis of BA indices in these samples provide valuable information for further BA metabolic disorder research.
开发并验证了一种简单、灵敏且可靠的定量和鉴定方法，用于同时分析人和啮齿动物（小鼠和大鼠）粪便样本中的58种胆汁酸（BA）。该方法包括用5%的乙醇氨水溶液进行提取步骤；通过高分辨率质谱（超高效液相色谱与四极杆-飞行时间质谱联用，UPLC-Q-TOF）对BA进行定量。三种生物样本的回收率为80.05-120.83%，变异系数（CV）为0.01-9.82%。检测限（LOD）在0.01-0.24μg/kg范围内，定量限（LOQ）在0.03-0.81μg/kg范围内。此外，该分析方法用于鉴定和定量终末期肾病（ESRD）患者、C57BL/6小鼠和Sprague-Dawley（SD）大鼠中的BA。这些样本中的粪便BA谱和BA指标分析为进一步的BA代谢紊乱研究提供了有价值的信息。
