Holert Johannes, Wilhelm Rudolf, Henker Jens, Reinker Claudia A, Müller Franziska M, Philipp Bodo
Institute for Molecular Microbiology and Biotechnology, Microbial Biotechnology & Ecology Group, University of Münster, Münster, Germany.
Dr. Falk Pharma GmbH, Freiburg, Germany.
Microb Biotechnol. 2025 Jun;18(6):e70178. doi: 10.1111/1751-7915.70178.
Ursodeoxycholic acid (UDCA) is widely administered to dissolve gallstones, treat liver disorders and reduce blood cholesterol levels. This study investigated fresh and dried chicken manure as a sustainable bioresource for chenodeoxycholic acid (CDCA), a precursor for the biotechnological production of UDCA. For this, bile acids from five commercial dried and seven fresh chicken manure samples were analysed. The bile acid pool consisted predominantly of CDCA (30%-90%) and 7-keto lithocholic acid (7k-LCA, 8%-56%), with minor amounts of cholic acid. CDCA concentrations varied between 62 and 2990 mg per kg dry weight, and the highest concentrations were found in two samples from fresh chicken manure, confirming that chickens can produce high but varying amounts of faecal CDCA. As a proof of principle, a newly created Pseudomonas putida KT2440 strain expressing a heterologous 7α-/7β-hydroxysteroid dehydrogenase system was shown to be able to transform manure-derived CDCA into UDCA without prior substrate purification from raw ethanolic chicken manure extracts. These results demonstrate that chicken manure can be used as an untapped resource for bile acids for biotechnological applications, providing a novel approach for the valorisation of this bioresource.
熊去氧胆酸（UDCA）被广泛用于溶解胆结石、治疗肝脏疾病以及降低血液胆固醇水平。本研究调查了新鲜和干燥鸡粪作为鹅去氧胆酸（CDCA）的可持续生物资源的潜力，CDCA是UDCA生物技术生产的前体。为此，分析了五个商业干燥鸡粪样品和七个新鲜鸡粪样品中的胆汁酸。胆汁酸池主要由CDCA（30%-90%）和7-酮石胆酸（7k-LCA，8%-56%）组成，胆酸含量较少。每千克干重中CDCA浓度在62至2990毫克之间，新鲜鸡粪的两个样品中浓度最高，这证实鸡能产生大量但含量各异的粪便CDCA。作为原理验证，新构建的表达异源7α-/7β-羟基类固醇脱氢酶系统的恶臭假单胞菌KT2440菌株被证明能够将粪便来源的CDCA转化为UDCA，而无需事先从粗乙醇鸡粪提取物中纯化底物。这些结果表明，鸡粪可作为一种未开发的胆汁酸生物资源用于生物技术应用，为这种生物资源的增值提供了一种新方法。
