Ravin Nikolai V, Rossetti Simona, Beletsky Alexey V, Kadnikov Vitaly V, Rudenko Tatyana S, Smolyakov Dmitry D, Moskvitina Marina I, Gureeva Maria V, Mardanov Andrey V, Grabovich Margarita Yu
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
Water Research Institute, IRSA-CNR, Monterotondo, 00185 Rome, Italy.
Microorganisms. 2022 Jun 27;10(7):1300. doi: 10.3390/microorganisms10071300.
The metagenome of foulings from sulfidic spring "Serovodorodny" (Tatarstan, Russia), where members of the genus was observed, was sequenced. Representatives of the phyla , and dominated in the microbial community. The complete genome of sp. KT was assembled from the metagenome. It displayed 93.93-99.72% 16S rRNA gene sequence identity to other species. The average nucleotide identity (ANI) и digital DNA-DNA hybridization (dDDH) showed that the genome designated KT represents a new species within the genus , ' Thiothrix sulfatifontis' sp. nov. KT. The taxonomic status has been determined of the strain sp. CT3, isolated about 30 years ago and not assigned to any of species due to high 16S rRNA gene sequence identity with related species (i.e., 98.8-99.4%). The complete genome sequence of strain CT3 was determined. The ANI between CT3 and other species was below 82%, and the dDDH values were less than 40%, indicating that strain CT3 belongs to a novel species, sp. nov. A genome analysis showed that both strains are chemo-organoheterotrophs, chemolithotrophs (in the presence of hydrogen sulfide and thiosulfate) and chemoautotrophs. For the first time, representatives of showed anaerobic growth in the presence of thiosulfate.
对来自俄罗斯鞑靼斯坦“Serovodorodny”硫化物泉污垢的宏基因组进行了测序，在该泉中观察到了某属的成员。厚壁菌门、变形菌门和拟杆菌门的代表在微生物群落中占主导地位。从宏基因组中组装出了某菌属KT菌株的完整基因组。它与其他某菌属物种的16S rRNA基因序列同一性为93.93 - 99.72%。平均核苷酸同一性（ANI）和数字DNA - DNA杂交（dDDH）表明基因组KT代表某菌属内的一个新物种，即“硫泉硫丝菌”新种KT。已确定了约30年前分离的CT3菌株的分类地位，由于其16S rRNA基因序列与相关物种具有高度同一性（即98.8 - 99.4%），之前未被归入任何某菌属物种。测定了CT3菌株的完整基因组序列。CT3与其他某菌属物种之间的ANI低于82%，dDDH值小于40%，表明CT3菌株属于一个新物种，即某菌属新种。基因组分析表明，这两个菌株都是化能有机异养菌、化能无机营养菌（在存在硫化氢和硫代硫酸盐的情况下）和化能自养菌。某菌属的代表首次在硫代硫酸盐存在的情况下表现出厌氧生长。
