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宏基因组学揭示了一个新属“脱硫硫杆菌属 dubininis”gen. nov.，sp. nov.和一个新种“硫丝菌 putei”sp. nov.，它们属于某些具有 Na-和 H-动力能量学特征的科。

Metagenomics Revealed a New Genus ' Thiocaldithrix dubininis' gen. nov., sp. nov. and a New Species ' Thiothrix putei' sp. nov. in the Family , Some Members of Which Have Traits of Both Na- and H-Motive Energetics.

机构信息

Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect, 33-2, 119071 Moscow, Russia.

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie Gory, 119991 Moscow, Russia.

出版信息

Int J Mol Sci. 2023 Sep 17;24(18):14199. doi: 10.3390/ijms241814199.

DOI:10.3390/ijms241814199
PMID:37762502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10532065/
Abstract

Two metagenome-assembled genomes (MAGs), GKL-01 and GKL-02, related to the family have been assembled from the metagenome of bacterial mat obtained from a sulfide-rich thermal spring in the North Caucasus. Based on average amino acid identity (AAI) values and genome-based phylogeny, MAG GKL-01 represented a new genus within the family. The GC content of the GKL-01 DNA (44%) differed significantly from that of other known members of the genus (50.1-55.6%). We proposed to assign GKL-01 to a new species and genus ' Thiocaldithrix dubininis' gen. nov., sp. nov. GKL-01. The phylogenetic analysis and estimated distances between MAG GKL-02 and the genomes of the previously described species of the genus allowed assigning GKL-02 to a new species with the proposed name ' Thiothrix putei' sp. nov. GKL-02 within the genus . Genome data first revealed the presence of both Na-ATPases and H-ATPases in several species. According to genomic analysis, bacteria GKL-01 and GKL-02 are metabolically versatile facultative aerobes capable of growing either chemolithoautotrophically or chemolithoheterotrophically in the presence of hydrogen sulfide and/or thiosulfate or chemoorganoheterotrophically.

摘要

已从北高加索富含硫的温泉细菌垫的宏基因组中组装了两个与 科相关的宏基因组组装基因组 (MAG)，GKL-01 和 GKL-02。基于平均氨基酸同一性 (AAI) 值和基于基因组的系统发育，MAG GKL-01 代表了 科内的一个新属。GKL-01 DNA 的 GC 含量 (44%) 与该属其他已知成员的 GC 含量 (50.1-55.6%) 有显著差异。我们建议将 GKL-01 分配到一个新的属和种“Thiocaldithrix dubininis”gen. nov.，sp. nov. GKL-01。MAG GKL-02 与先前描述的属内种的基因组之间的系统发育分析和估计距离允许将 GKL-02 分配到一个新种，命名为“Thiothrix putei”sp. nov. GKL-02，属内 。基因组数据首次揭示了几种 物种中均存在 Na-ATP 酶和 H-ATP 酶。根据基因组分析，细菌 GKL-01 和 GKL-02 是代谢多功能的兼性需氧菌，能够在存在硫化氢和/或硫代硫酸盐或化能有机异养的情况下，要么化能自养生长，要么化能异养生长。

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