Bello Sarah, Howard-Azzeh Mohammad, Schellhorn Herb E, Gupta Radhey S
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8N 3Z5, Canada.
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
Microorganisms. 2022 Jun 29;10(7):1312. doi: 10.3390/microorganisms10071312.
Evolutionary relationships amongst and species/strains were examined using phylogenomic and comparative analyses of genome sequences. In a phylogenomic tree based on 282 conserved proteins, the named species formed a monophyletic clade containing two distinct subclades. One clade, encompassing the genera and corresponds to the family whereas another clade, harboring Thermochlorobacter aerophilum, Thermochlorobacteriaceae bacterium GBChlB, and sp. 445, is now proposed as a new family ( fam. nov). In parallel, our comparative genomic analyses have identified 47 conserved signature indels (CSIs) in diverse proteins that are exclusively present in members of the class or its two families, providing reliable means for identification. Two known species in our phylogenomic tree are found to group within a larger clade containing several species and uncultured strains. A CSI in the SecY protein is uniquely shared by the species/strains from this "larger clade". Two additional CSIs, which are commonly shared by species and the "larger clade", support a specific relationship between these two groups. The newly identified molecular markers provide novel tools for genetic and biochemical studies and identification of these organisms.
利用基因组序列的系统发育基因组学和比较分析，研究了各物种/菌株之间的进化关系。在基于282种保守蛋白构建的系统发育树中，命名的物种形成了一个单系分支，包含两个不同的亚分支。一个分支包含属和属，对应于科，而另一个分支包含嗜热嗜氯杆菌、嗜热嗜氯杆菌科细菌GBChlB和种445，现在被提议作为一个新科（新科）。同时，我们的比较基因组分析在多种蛋白质中鉴定出47个保守特征插入/缺失（CSIs），这些特征仅存在于类或其两个科的成员中，为鉴定提供了可靠的方法。在我们的系统发育树中，发现两个已知的物种聚集在一个更大的分支中，该分支包含几个物种和未培养的菌株。来自这个“更大的分支”的物种/菌株独特地共享SecY蛋白中的一个CSI。另外两个CSIs，通常由物种和“更大的分支”共享，支持这两组之间的特定关系。新鉴定的分子标记为这些生物体的遗传和生化研究及鉴定提供了新工具。
