Stoakes Emily, Savva George M, Coates Ruby, Tejera Noemi, Poolman Mark G, Grant Andrew J, Wain John, Singh Dipali
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK.
Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK.
Microorganisms. 2022 Jul 5;10(7):1355. doi: 10.3390/microorganisms10071355.
, the major cause of bacterial foodborne illness, is also a fastidious organism that requires strict growth requirements in the laboratory. Our aim was to study substrate utilisation and energy metabolism in non-growing to investigate the ability of these bacteria to survive so effectively in the food chain. We integrated phenotypic microarrays and genome-scale metabolic modelling (GSM) to investigate the survival of on 95 substrates. We further investigated the underlying metabolic re-adjustment associated with varying energy demands on each substrate. We identified amino acids, organic acids and H2, as single substrates supporting survival without growth. We identified several different mechanisms, which were used alone or in combination, for ATP production: substrate-level phosphorylation via acetate kinase, the TCA cycle, and oxidative phosphorylation via the electron transport chain that utilised alternative electron donors and acceptors. The benefit of ATP production through each of these mechanisms was associated with the cost of enzyme investment, nutrient availability and/or O2 utilisation. can utilise a wide range of substrates as energy sources, including organic acids commonly used for marination or preservation of ingredients, which might contribute to the success of their survival in changing environments.
作为细菌性食源性疾病的主要病因，也是一种苛求菌，在实验室中需要严格的生长条件。我们的目的是研究非生长状态下的底物利用和能量代谢，以调查这些细菌在食物链中如此有效生存的能力。我们整合了表型微阵列和基因组规模代谢建模（GSM）来研究在95种底物上的生存情况。我们进一步研究了与每种底物上不同能量需求相关的潜在代谢重新调整。我们确定氨基酸、有机酸和H2作为支持无生长存活的单一底物。我们确定了几种单独或组合使用的ATP产生机制：通过乙酸激酶的底物水平磷酸化、三羧酸循环以及通过利用替代电子供体和受体的电子传递链进行的氧化磷酸化。通过这些机制中每一种产生ATP的益处与酶投入成本、营养可用性和/或氧气利用相关。可以利用多种底物作为能源，包括常用于腌制或保存食材的有机酸，这可能有助于它们在不断变化的环境中成功生存。
