Tewes Thomas J, Centeleghe Isabella, Maillard Jean-Yves, Platte Frank, Bockmühl Dirk P
Faculty of Life Sciences, Rhine-Waal University of Applied Sciences, Marie-Curie-Straße 1, 47533 Kleve, Germany.
School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff CF10 3NB, Wales, UK.
Microorganisms. 2022 Jul 7;10(7):1369. doi: 10.3390/microorganisms10071369.
Moist/hydrated biofilms have been well-studied in the medical area, and their association with infections is widely recognized. In contrast, dry-surface biofilms (DSBs) on environmental surfaces in healthcare settings have received less attention. DSBs have been shown to be widespread on commonly used items in hospitals and to harbor bacterial pathogens that are known to cause healthcare-acquired infections (HAI). DSBs cannot be detected by routine surface swabbing or contact plates, and studies have shown DSBs to be less susceptible to cleaning/disinfection products. As DSBs are increasingly reported in the medical field, and there is a likelihood they also occur in food production and manufacturing areas, there is a growing demand for the rapid in situ detection of DSBs and the identification of pathogens within DSBs. Raman microspectroscopy allows users to obtain spatially resolved information about the chemical composition of biofilms, and to identify microbial species. In this study, we investigated mono-species DSB on polyvinylchloride blanks and stainless steel coupons, and dual-species (/) DSB on steel coupons. We demonstrated that Raman microspectroscopy is not only suitable for identifying specific species, but it also enables the differentiation of vegetative cells from their sporulated form. Our findings provide the first step towards the rapid identification and characterization of the distribution and composition of DSBs on different surface areas.
潮湿/水合生物膜在医学领域已得到充分研究，其与感染的关联也已得到广泛认可。相比之下，医疗环境中环境表面的干表面生物膜（DSB）受到的关注较少。研究表明，DSB在医院常用物品上广泛存在，并携带已知会导致医疗保健相关感染（HAI）的细菌病原体。常规表面擦拭或接触平板无法检测到DSB，且研究表明DSB对清洁/消毒产品的敏感性较低。随着DSB在医学领域的报道越来越多，并且它们也有可能出现在食品生产和制造区域，对DSB的快速原位检测以及DSB内病原体的鉴定的需求日益增长。拉曼光谱显微镜使用户能够获得有关生物膜化学成分的空间分辨信息，并识别微生物种类。在本研究中，我们调查了聚氯乙烯空白样品和不锈钢试片上的单物种DSB，以及钢试片上的双物种（/）DSB。我们证明拉曼光谱显微镜不仅适用于识别特定物种，还能区分营养细胞与其芽孢形式。我们的研究结果为快速识别和表征不同表面积上DSB的分布和组成迈出了第一步。
