Ratnayake Dharanga, Ansah Michael, Al Ani Enas, Heaselgrave Wayne
Department of Biomedical Science, University of Wolverhampton, Wolverhampton WV1 1LY, UK.
Research Institute in Healthcare Science, University of Wolverhampton, Wolverhampton WV1 1LY, UK.
Microorganisms. 2022 Jul 8;10(7):1375. doi: 10.3390/microorganisms10071375.
In recent years, a rise in the number of contact lens users in the UK and worldwide coincided with an increased incidence of microbial keratitis. The aim of this study was to investigate the antimicrobial activities of polyhexamethylene guanidine (PHMG), polyaminopropyl biguanide (PAPB), and guazatine in comparison to the common contact lens disinfectant constituent, polyhexamethylene biguanide (PHMB). The study investigated these compounds against a broad range of organisms, including Acanthamoeba castellanii, Acanthamoeba polyphaga, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. This study demonstrated that PHMG, PAPB, and guazatine are equal in activity to PHMB against Acanthamoeba trophozoites and cysts. PHMG and PAPB are also equal in activity to PHMB against S. aureus and P. aeruginosa, whereas PHMG shows significantly better activity than PHMB against C. albicans (p < 0.001). To our knowledge, this is the first study to demonstrate the effectiveness of PHMB, PHMG, PAPB, and guazatine against Acanthamoeba and other ocular pathogens. As alternatives to PHMB, these compounds warrant further investigation for inclusion in contact lens solutions and for the treatment of keratitis.
近年来，英国及全球范围内隐形眼镜使用者数量的增加与微生物性角膜炎发病率的上升同时出现。本研究的目的是比较聚六亚甲基胍（PHMG）、聚氨基丙基双胍（PAPB）和胍杀定与常见隐形眼镜消毒剂成分聚六亚甲基双胍（PHMB）的抗菌活性。该研究调查了这些化合物对多种生物体的作用，包括卡氏棘阿米巴、多食棘阿米巴、金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌。本研究表明，PHMG、PAPB和胍杀定对棘阿米巴滋养体和包囊的活性与PHMB相当。PHMG和PAPB对金黄色葡萄球菌和铜绿假单胞菌的活性也与PHMB相当，而PHMG对白色念珠菌的活性显著优于PHMB（p<0.001）。据我们所知，这是第一项证明PHMB、PHMG、PAPB和胍杀定对棘阿米巴和其他眼部病原体有效的研究。作为PHMB的替代品，这些化合物值得进一步研究，以纳入隐形眼镜护理液和用于治疗角膜炎。
