Castañeda Alejo Sarelia M, Tejada Meza Kevin, Valderrama Valencia María R, Arenazas Rodríguez Armando J, Málaga Espinoza Christian J
Departamento Académico de Arquitectura e Ingenierías Civil y del Ambiente, Universidad Católica de Santa María-UCSM, Urb. San José, San Jose S/n, Yanahuara, Arequipa 04000, Peru.
Departamento Académico de Farmacia, Bio-Química y Biotecnología, Universidad Católica de Santa María-UCSM, Urb. San José, San Jose S/n, Yanahuara, Arequipa 04000, Peru.
Microorganisms. 2022 Jul 14;10(7):1414. doi: 10.3390/microorganisms10071414.
Rubber is a natural product, the main car tire component. Due to the characteristics acquired by this material after its vulcanization process, its degradation under natural conditions requires very long times, causing several environmental problems. In the present work, the existence of a bacterial consortium isolated from a discarded tire found within the Socabaya River with the ability to degrade shredded tire rubber without any chemical pretreatment is explored. Taking into consideration the complex chemical composition of a rubber tire and the described benefits of the use of pretreatments, the study is developed as a preliminary analysis. The augmentative growth technique was used, and the level of degradation was quantified as a percentage through the analysis of microbial respiration. Schiff's test and the use of comparative photographs of scanning electron microscopy (SEM) were also used. The consortium using next generation genetic sequencing was analyzed. A 4.94% degradation point was obtained after 20 days of experimentation, and it was found that the consortium was mostly made up with with 69.12% of the total genetic readings of the consortium and the existence of 15% of unidentified microbial strains at the genre level. The role played by the organisms in the degradation process is unknown. However, the positive results in the tests carried out show that the consortium had action on the shredded tire, showing a mineralization process.
橡胶是一种天然产品，是汽车轮胎的主要组成部分。由于这种材料在硫化过程后所具有的特性，其在自然条件下的降解需要很长时间，从而引发了若干环境问题。在本研究中，探索了从索卡巴亚河中发现的一个废弃轮胎中分离出的一个细菌群落，该群落能够在不进行任何化学预处理的情况下降解切碎的轮胎橡胶。考虑到橡胶轮胎复杂的化学成分以及使用预处理的既定益处，该研究作为初步分析开展。采用了增殖生长技术，并通过微生物呼吸分析将降解水平量化为百分比。还使用了席夫氏试验以及扫描电子显微镜（SEM）的对比照片。对使用下一代基因测序的群落进行了分析。经过20天的实验，获得了4.94%的降解率，并且发现该群落主要由占群落总基因读数69.12%的[此处原文有误，多了个with]组成，在属水平上存在15%的未鉴定微生物菌株。这些生物体在降解过程中所起的作用尚不清楚。然而，所进行的测试中的积极结果表明，该群落对切碎的轮胎有作用，显示出一个矿化过程。
