Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry, Lodz University of Technology, Lodz, Poland.
Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry, Lodz University of Technology, Lodz, Poland.
J Hazard Mater. 2019 Apr 5;367:8-14. doi: 10.1016/j.jhazmat.2018.12.045. Epub 2018 Dec 17.
Aniline-degrading yeast strain - Candida methanosorbosa BP-6 was tested for its ability to degrade ground tire rubber, treated and non-treated with ozone. The protein content, respiratory activity, critical oxygen concentration (COC) and emulsifying activity of the yeast strain were monitored during 21 day degradation process. The effects of biodegradation were evaluated using aldehyde detection, Scanning Electrone Microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR) analysis. Pre-treatment of ground tire rubber with ozone resulted in lower microbial growth. However, metabolic condition of the C. methanosorbosa BP-6 yeast strain was higher in sample with ozonized tire rubber. Furthermore, the COC values in the last days of the process were about 30% lower regarding non-ozonized polymer. Also, the ozonization of tire rubber resulted in higher biosurfactant production of the yeast strain. The roughness and visible gaps in rubber matrix (SEM analysis) confirmed the ability of Candida methanosorbosa BP-6 yeast strain for tire rubber biodegradation.
苯胺降解酵母菌株-甲醇营养型毕赤酵母 BP-6 被测试其降解经过和未经臭氧处理的废旧轮胎的能力。在 21 天的降解过程中，监测了酵母菌株的蛋白质含量、呼吸活性、临界需氧量 (COC) 和乳化活性。使用醛检测、扫描电子显微镜 (SEM) 和傅里叶变换红外光谱 (FTIR) 分析来评估生物降解的效果。臭氧预处理废旧轮胎会导致微生物生长减少。然而，在臭氧化轮胎橡胶样品中，甲醇营养型毕赤酵母 BP-6 酵母菌株的代谢条件更高。此外，在实验过程的最后几天，非臭氧化聚合物的 COC 值降低了约 30%。而且，臭氧化轮胎橡胶会导致酵母菌株产生更多的生物表面活性剂。橡胶基质的粗糙度和可见间隙（SEM 分析）证实了甲醇营养型毕赤酵母 BP-6 酵母菌株对轮胎橡胶生物降解的能力。
