Institut de Chimie, Université de Neuchâtel, Avenue de Bellevaux 51, CH-2000 Neuchâtel, Switzerland.
Laboratoire PEIRENE UR 22722, Faculté de Pharmacie, Université de Limoges, F-87025 Limoges, France.
Molecules. 2022 Jul 13;27(14):4472. doi: 10.3390/molecules27144472.
The effect of dimethyl sulfoxide (DMSO) in rheumatoid arthritis (RA) human fibroblast-like synoviocytes (FLSs) has been studied on five different samples harvested from the joints (fingers, hands and pelvis) of five women with RA. At high concentrations (>5%), the presence of DMSO induces the cleavage of caspase-3 and PARP-1, two phenomena associated with the cell death mechanism. Even at a 0.5% concentration of DMSO, MTT assays show a strong toxicity after 24 h exposure (≈25% cell death). Therefore, to ensure a minimum impact of DMSO on RA FLSs, our study shows that the concentration of DMSO has to be below 0.05% to be considered safe.
已在五个患有类风湿关节炎（RA）的女性的关节（手指、手和骨盆）中采集的五个不同样本上研究了二甲基亚砜（DMSO）对类风湿关节炎成纤维样滑膜细胞（FLS）的影响。在高浓度（>5％）下，DMSO 的存在会诱导半胱天冬酶-3 和 PARP-1 的裂解，这两种现象与细胞死亡机制有关。即使 DMSO 的浓度为 0.5％，MTT 测定在 24 小时暴露后（≈25％的细胞死亡）也显示出很强的毒性。因此，为了确保 DMSO 对 RA FLS 的影响最小，我们的研究表明 DMSO 的浓度必须低于 0.05％才能被认为是安全的。
