Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad 38040, Pakistan.
Molecules. 2022 Jul 18;27(14):4582. doi: 10.3390/molecules27144582.
In the current study, the anti-inflammatory and analgesic potential of Alnus nitida (leaves and fruits) was evaluated in the Sprague-Dawley rat. Traditionally, A. nitida was used for the treatment of inflammatory ailments. However, A. nitida leaves and fruits have not been yet reported regarding any potential medicinal effects. Leaves/fruits of A. nitida were extracted with methanol and fractionated to attain n-hexane, chloroform, ethyl acetate and aqueous fractions. These extracts were then evaluated for in vivo analgesic and anti-inflammatory potential. For in vivo anti-inflammatory activity, carrageenan-induced paw edema assay, Freunds’ complete adjuvant-induced edema, xylene-induced ear edema and histamine-induced paw edema models were used in rats, which showed significant (p < 0.01) reduction (70−80%) in edema in comparison of inflammatory controls. On other hand, for the analgesic assessment, hot plate assay and acetic acid-induced writhing tests were used, which showed a significant (p < 0.01) rise in latency time (40−60%) as compared with pain-induced controls. These results were comparable with standard drugs in a concentration-dependent manner and no mortality or toxicity was observed during all experiments. Then, for the identification of chemical constituents gas chromatography−mass spectrometry (GC-MS) analysis was performed, which indicated the presence of neophytadiene, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, phytol and vitamin E, justifying the use of A. nitida to treat inflammatory disorders.
在目前的研究中，评估了桦木（叶和果实）在 Sprague-Dawley 大鼠中的抗炎和镇痛潜力。传统上，桦木被用于治疗炎症性疾病。然而，尚未有关于桦木叶和果实具有任何潜在药用功效的报道。桦木叶和果实用甲醇提取，然后分馏得到正己烷、氯仿、乙酸乙酯和水相。然后评估这些提取物的体内镇痛和抗炎潜力。为了进行体内抗炎活性研究，使用角叉菜胶诱导的爪肿胀试验、完全弗氏佐剂诱导的肿胀、二甲苯诱导的耳肿胀和组胺诱导的爪肿胀模型，与炎症对照组相比，这些提取物显著（p<0.01）减少了 70-80%的肿胀。另一方面，为了进行镇痛评估，使用热板试验和醋酸诱导的扭体试验，与疼痛诱导对照组相比，潜伏期显著（p<0.01）延长了 40-60%。这些结果与标准药物呈浓度依赖性，在所有实验过程中均未观察到死亡或毒性。然后，进行气相色谱-质谱（GC-MS）分析以鉴定化学成分，结果表明存在新 Phytadiene、3,7,11,15-四甲基-2-十六碳烯-1-醇、叶绿醇和维生素 E，这证明了桦木用于治疗炎症性疾病的合理性。
