College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
College of Science, Sichuan Agricultural University, Ya'an 625000, China.
Molecules. 2022 Jul 19;27(14):4589. doi: 10.3390/molecules27144589.
The separation of chemical components from wild plants to develop new pesticides is a hot topic in current research. To evaluate the antimicrobial effects of metabolites of Ligusticum chuanxiong (CX), we systematically studied the antimicrobial activity of extracts of CX, and the active compounds were isolated, purified and structurally identified. The results of toxicity measurement showed that the extracts of CX had good biological activities against Botrytis cinerea, Sclerotinia sclerotiorum, Alternaria alternata and Pythium aphanidermatum, and the value of EC50 were 130.95, 242.36, 332.73 and 307.29 mg/L, respectively. The results of in vivo determination showed that under the concentration of 1000 mg/L, the control effect of CX extract on Blumeria graminis was more than 40%, and the control effect on Botrytis cinerea was 100%. The antifungal active components of CX were identified as Senkyunolide A and Ligustilide by mass spectrometry and nuclear magnetic resonance. The MIC (minimum inhibitory concentration) value of Senkyunolide A and Ligustilide against Fusarium graminearum were 7.81 and 62.25 mg/L, respectively. As a new botanical fungicide with a brightly exploitative prospect, CX extract has potential research value in the prevention and control of plant diseases.
从野生植物中分离化学物质来开发新的农药是当前研究的热门话题。为了评估藁本（川芎）代谢物的抗菌效果，我们系统地研究了川芎提取物的抗菌活性，并对活性化合物进行了分离、纯化和结构鉴定。毒性测量结果表明，川芎提取物对灰葡萄孢、核盘菌、链格孢和腐霉具有良好的生物活性，EC50 值分别为 130.95、242.36、332.73 和 307.29mg/L。体内测定结果表明，在 1000mg/L 浓度下，川芎提取物对小麦白粉病的防治效果超过 40%，对灰霉病的防治效果为 100%。通过质谱和核磁共振鉴定，川芎的抗真菌活性成分为川芎嗪 A 和川芎内酯。Senkyunolide A 和 Ligustilide 对禾谷镰刀菌的最小抑菌浓度（MIC）值分别为 7.81 和 62.25mg/L。作为一种具有广阔开发前景的新型植物源杀菌剂，川芎提取物在植物病害防治方面具有潜在的研究价值。
