College of Agronomy, Sichuan Agricultural University, Chengdu, China.
Chengdu TePu Biotech Co., Ltd, Chengdu, China.
Pest Manag Sci. 2021 Oct;77(10):4375-4382. doi: 10.1002/ps.6471. Epub 2021 May 20.
Wheat powdery mildew is a worldwide fungal disease and one of the main diseases harming wheat production. Bacillus subtilis is a vital biocontrol bacteria with broad-spectrum antimicrobial activity. In this study, we systematically studied the control effect of B. subtilis on wheat powdery mildew.
The control efficiency of 4 × 10 CFU ml B. subtilis on wheat leaves was 71.75% in vitro and 70.31% in a pot experiment. Application of 4 × 10 CFU ml B. subtilis significantly inhibited spore germination (spore germination rate of 22.23%) and increased appressorium deformity (appressorium deformity rate of 69.33%). This was significantly different from the results in the sterile water treatment. Through transcriptome sequencing analysis, we found that differentially expressed genes were mainly enriched in the biosynthesis and metabolism of amino acids (including phenylalanine), carbon metabolism, the pentose phosphate pathway and other pathways. In particular, the plant hormone signal pathway gene nonexpressor of pathogenesis-related genes 1 (NPR1) was significantly upregulated.
B. subtilis at concentrations of 4 × 10 CFU ml had a significant control effect on wheat powdery mildew and can inhibit germination of the conidial germ tubes and the normal development of appressorium. B. subtilis may induce disease resistance in wheat to control wheat powdery mildew, and this effect is related to the salicylic acid-dependent signal pathway. © 2021 Society of Chemical Industry.
小麦白粉病是一种世界性真菌病害，也是危害小麦生产的主要病害之一。枯草芽孢杆菌是一种具有广谱抗菌活性的重要生防细菌。本研究系统研究了枯草芽孢杆菌对小麦白粉病的防治效果。
4×10 CFU/ml 的枯草芽孢杆菌对离体小麦叶片的防治效率为 71.75%，盆栽试验中的防治效率为 70.31%。施用 4×10 CFU/ml 的枯草芽孢杆菌可显著抑制孢子萌发（孢子萌发率为 22.23%），增加附着胞变形（附着胞变形率为 69.33%）。这与无菌水处理的结果有显著差异。通过转录组测序分析，发现差异表达基因主要富集在氨基酸（包括苯丙氨酸）、碳代谢、戊糖磷酸途径等途径的生物合成和代谢。特别是植物激素信号通路基因非致病性相关基因 1（NPR1）显著上调。
浓度为 4×10 CFU/ml 的枯草芽孢杆菌对小麦白粉病具有显著的防治效果，可抑制分生孢子芽管的萌发和附着胞的正常发育。枯草芽孢杆菌可能通过诱导小麦的抗病性来控制小麦白粉病，这种作用与水杨酸依赖的信号通路有关。 © 2021 化学工业协会。
