Department of Physiology, School of Medicine, Daegu Catholic University, Daegu 42472, Korea.
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea.
Molecules. 2022 Jul 20;27(14):4633. doi: 10.3390/molecules27144633.
Citropten is a coumarin that is mainly found in fruits of Rutaceae trees, but its anti-inflammatory activities in colitis is still unknown. In this study, we investigated its attenuating effect of citropten isolated from extract on DSS-induced colitis through the modulation of the activity of T cells and intestinal epithelial cells. We found that pre-treatment with citropten downregulates the activity of T cells and intestinal epithelial cells without a negative effect on the viability of Jurkat and HT-29 cells. The results from the Western blot analysis revealed that pre-treatment with citropten reduces the NFκB and MAPK signaling pathway in activated T cells and intestinal epithelial cells. We elucidated that the oral administration of citropten alleviates the colonic inflammation and activity of effector T cells in DSS-induced colitis by measuring changes in body weight, histological scoring from H&E-stained sections, mRNA levels of pro-inflammatory cytokines and the phosphorylation level of the MAPK signaling pathway.
枸橼苦素是一种香豆素，主要存在于芸香科树木的果实中，但它在结肠炎中的抗炎活性尚不清楚。在这项研究中，我们通过调节 T 细胞和肠上皮细胞的活性，研究了从 提取物中分离得到的枸橼苦素对 DSS 诱导的结肠炎的缓解作用。我们发现，枸橼苦素预处理可下调 T 细胞和肠上皮细胞的活性，而对 Jurkat 和 HT-29 细胞的活力无不良影响。Western blot 分析结果表明，枸橼苦素预处理可降低活化 T 细胞和肠上皮细胞中 NFκB 和 MAPK 信号通路的活性。我们通过测量体重变化、H&E 染色切片的组织学评分、促炎细胞因子的 mRNA 水平和 MAPK 信号通路的磷酸化水平，阐明了枸橼苦素的口服给药可通过减轻 DSS 诱导的结肠炎中的结肠炎症和效应 T 细胞的活性。
