Margaret Ritchie School of Family and Consumer Sciences, University of Idaho, Moscow, ID 83844, USA.
Nutrients. 2022 Jul 6;14(14):2787. doi: 10.3390/nu14142787.
Little is known about the impact of human colostrum on infant intestinal health following digestion. The aim of this study was to compare the effect of digested versus undigested human colostrum on inflammation and cytotoxicity in human intestinal epithelial cells (Caco2BBe) stimulated with lipopolysaccharides (LPS) or tumor necrosis factor (TNF). Colostrum samples (days 2-8 postpartum) from ten mothers of preterm infant were applied. Caco2BBe cells were pretreated by digested or undigested colostrum before stimulation with LPS or TNF. The inflammatory response was determined by measuring the production of interleukin-8 (IL-8) from cells using enzyme linked immunosorbent assay (ELISA). Cytotoxicity was examined by measuring the release of lactate dehydrogenase (LDH) from the cells. Digested colostrum significantly reduced IL-8 production under LPS and TNF stimulation compared with undigested colostrum. Individual colostrum samples exhibited wide variance in the ability to suppress IL-8 production and cytotoxicity in Caco2BBe cells. In vitro-digested human colostrum suppressed an inflammatory response more than undigested human colostrum in an induced intestinal cell culture model.
关于人初乳在消化后对婴儿肠道健康的影响知之甚少。本研究旨在比较经消化和未经消化的人初乳对脂多糖（LPS）或肿瘤坏死因子（TNF）刺激的人肠上皮细胞（Caco2BBe）的炎症和细胞毒性的影响。使用了来自 10 位早产儿母亲的初乳样本（产后第 2-8 天）。在 LPS 或 TNF 刺激之前，用消化或未消化的初乳预处理 Caco2BBe 细胞。通过酶联免疫吸附试验（ELISA）测量细胞中白细胞介素-8（IL-8）的产生来确定炎症反应。通过测量细胞中乳酸脱氢酶（LDH）的释放来检查细胞毒性。与未消化的初乳相比，消化后的初乳在 LPS 和 TNF 刺激下显著降低了 IL-8 的产生。个体初乳样本在抑制 Caco2BBe 细胞中 IL-8 产生和细胞毒性的能力方面表现出很大的差异。在诱导的肠道细胞培养模型中，体外消化的人初乳比未消化的人初乳更能抑制炎症反应。
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