Nutrition Program, School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USA.
Department of Pediatrics, University of California, Davis, Sacramento, CA 95817, USA.
Nutrients. 2018 May 17;10(5):631. doi: 10.3390/nu10050631.
Human milk provides immunoglobulins (Igs) that supplement the passive immune system of neonates; however, the extent of survival of these Igs during gastric digestion and whether this differs between preterm and term infants remains unknown. Human milk, and infant gastric samples at 2 h post-ingestion were collected from 15 preterm (23⁻32 week gestational age (GA)) mother-infant pairs and from 8 term (38⁻40 week of GA) mother-infant pairs within 7⁻98 days postnatal age. Samples were analyzed via ELISA for concentration of total IgA (secretory IgA (SIgA)/IgA), total secretory component (SC/SIgA/SIgM), total IgM (SIgM/IgM), and IgG as well as peptidomics. Total IgA concentration decreased by 60% from human milk to the preterm infant stomach and decreased by 48% in the term infant stomach. Total IgM and IgG concentrations decreased by 33% and 77%, respectively, from human milk to the term infant stomach but were stable in the preterm infant stomach. Release of peptides from all Ig isotypes in the term infant stomach was higher than in the preterm stomach. Overall, the stability of human milk Igs during gastric digestion is higher in preterm infant than in term infants, which could be beneficial for assisting the preterm infants' immature immune system.
人乳提供免疫球蛋白（Igs），补充新生儿的被动免疫系统；然而，这些 Igs 在胃消化过程中的存活程度以及早产儿和足月儿之间是否存在差异尚不清楚。从 15 对早产儿（23-32 周胎龄（GA））母婴和 8 对足月儿（38-40 周 GA）母婴中收集人乳和婴儿摄入后 2 小时的胃样本。通过 ELISA 分析总 IgA（分泌型 IgA（SIgA）/IgA）、总分泌成分（SC/SIgA/SIgM）、总 IgM（SIgM/IgM）和 IgG 的浓度以及肽组学。总 IgA 浓度从人乳降至早产儿胃中降低了 60%，在足月儿胃中降低了 48%。总 IgM 和 IgG 浓度分别从人乳降至足月儿胃中降低了 33%和 77%，但在早产儿胃中稳定。所有 Ig 同种型的肽从足月婴儿胃中释放的量高于早产儿胃。总的来说，人乳 Igs 在胃消化过程中的稳定性在早产儿中高于足月儿，这可能有助于辅助早产儿不成熟的免疫系统。
