Ho Jou-Hsuan, Baskaran Rathinasamy, Wang Ming-Fu, Yang Hong-Siang, Lo Yun-Hsin, Mohammedsaleh Zuhair M, Lin Wan-Teng
Department of Food Science, Tunghai University, Taichung 407705, Taiwan.
Department of Bioinformatics and Medical Engineering, Asia University, Taichung 413305, Taiwan.
Pharmaceuticals (Basel). 2022 Jul 1;15(7):819. doi: 10.3390/ph15070819.
Peptides are fragments of fundamental protein sequences that may have health benefits in addition to basic dietary benefits. Recently, we have reported on the pharmacological benefits of alcalase potato protein hydrolysate (APPH) and bioactive peptides isolated from APPH. The aim was to evaluate the synergistic effect of exercise along with DIKTNKPVIF (DF) peptides in ameliorating hypertension in spontaneously hypertensive rat (SHR) rats. We examined ECG parameters, lipid profiles, cardiac markers, and histology, and quantified the proteins associated with fibrosis, hypertrophy, apoptosis, mitochondrial biogenesis, and longevity pathways. DF peptide administration, along with exercise, reduced the blood pressure and cardiac marker levels in serum. Furthermore, it also suppressed the expression of fibrosis markers COL1A1, CTGF, and uPA and downregulated cardiac-hypertrophy-associated markers such as calcineurin, NFATC3, GATA4, pGATA4 and BNP. Exercise synergistically increases the expression of IFG1, PI3K, and AKT cell-survival pathway proteins, along with DF administration. Moreover, AMPK/SIRT1/PGC-1α/FOXO3 pathway protein expression was increased with the combinatorial administration of DF and exercise. Our data suggest that exercise, along with DF peptides, act synergistically in alleviating hypertension by activating the mitochondrial biogenesis pathway.
肽是基本蛋白质序列的片段，除了具有基本的饮食益处外，可能还对健康有益。最近，我们报道了碱性蛋白酶水解马铃薯蛋白（APPH）及其分离出的生物活性肽的药理益处。本研究旨在评估运动与DIKTNKPVIF（DF）肽协同改善自发性高血压大鼠（SHR）高血压的效果。我们检测了心电图参数、血脂谱、心脏标志物和组织学，并对与纤维化、肥大、凋亡、线粒体生物发生和长寿途径相关的蛋白质进行了定量分析。给予DF肽并结合运动，可降低血清中的血压和心脏标志物水平。此外，它还抑制了纤维化标志物COL1A1、CTGF和uPA的表达，并下调了与心脏肥大相关的标志物，如钙调神经磷酸酶、NFATC3、GATA4、磷酸化GATA4和脑钠肽。运动与DF肽联合使用可协同增加IFG1、PI3K和AKT细胞存活通路蛋白的表达。此外，DF与运动联合给药可增加AMPK/SIRT1/PGC-1α/FOXO3通路蛋白的表达。我们的数据表明，运动与DF肽通过激活线粒体生物发生途径协同作用，减轻高血压。
