Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Department of Nutrition and Food Hygiene, College of Public Health, Inner Mongolia Medical University, Hohhot 010059, China.
Nutrients. 2022 Dec 12;14(24):5289. doi: 10.3390/nu14245289.
The physicochemical properties and multiple bioactive effects of ginseng oligopeptides (GOPs), plant-derived small molecule bioactive peptides, suggest a positive influence on health span and longevity. Given this, cellular senescence is the initiating factor and key mechanism of aging in the organism, and thus the current study sought to explore the effects of GOPs on HO-induced cellular senescence and its potential mechanisms. Senescence was induced in mouse embryonic fibroblasts NIH/3T3 by 4 h of exposure to 200 µM HO and confirmed using CCK-8 assay and Western blot analyses of p16 and p21 after 24 h of growth medium administration with or without GOPs supplementation (25, 50, and 100 µg/mL). We found that GOPs delayed oxidative stress-induced NIH/3T3 senescence by inhibiting the G1 phase arrest, increasing DNA synthesis in the S phase, decreasing the relative protein expression of p16 and p21, promoting cell viability, protecting DNA, and enhancing telomerase (TE) activity. Further investigation revealed that the increase in antioxidative capacity and anti-inflammation capacity might form the basis for the retarding of the senescence effects of GOPs. Furthermore, GOPs supplementation significantly improved mitochondrial function and mitochondrial biogenesis via the NAD/SIRT1/PGC-1𝛼 pathway. These findings indicate that GOPs may have a positive effect on health span and lifespan extension via combating cellular senescence, oxidative stress, and inflammation, as well as modulating longevity regulating pathway NAD/SIRT1/PGC-1𝛼.
人参寡肽（GOPs）是一种源自植物的小分子生物活性肽，具有多种生物活性，其理化性质表明它对健康寿命和长寿有积极影响。鉴于此，细胞衰老被认为是生物体衰老的起始因素和关键机制，因此本研究旨在探讨 GOPs 对 HO 诱导的细胞衰老及其潜在机制的影响。通过将小鼠胚胎成纤维细胞 NIH/3T3 暴露于 200µM HO 中 4 小时，诱导衰老，并用 CCK-8 测定法和 Western blot 分析在生长培养基中添加或不添加 GOPs（25、50 和 100µg/mL）24 小时后 p16 和 p21 的表达来确认衰老。结果发现，GOPs 通过抑制 G1 期阻滞、增加 S 期 DNA 合成、降低 p16 和 p21 的相对蛋白表达、促进细胞活力、保护 DNA 和增强端粒酶（TE）活性，延缓氧化应激诱导的 NIH/3T3 衰老。进一步研究表明，抗氧化能力和抗炎能力的增强可能是 GOPs 延缓衰老作用的基础。此外，GOPs 补充通过 NAD/SIRT1/PGC-1α 途径显著改善线粒体功能和线粒体生物发生。这些发现表明，GOPs 可能通过对抗细胞衰老、氧化应激和炎症以及调节长寿调节途径 NAD/SIRT1/PGC-1α 对健康寿命和寿命延长产生积极影响。
