Jailani Afreen, Kalimuthu Shanthini, Rajasekar Vidhyashree, Ghosh Sumanta, Collart-Dutilleul Pierre-Yves, Fatima Naveen, Koo Hyun, Solomon Adline Princy, Cuisinier Frederic, Neelakantan Prasanna
Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China.
Quorum Sensing Laboratory, Centre for Research in Infectious Diseases, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613401, India.
Pharmaceutics. 2022 Jul 7;14(7):1428. doi: 10.3390/pharmaceutics14071428.
Dental caries, a preventable disease, is caused by highly-adherent, acid-producing biofilms composed of bacteria and yeasts. Current caries-preventive approaches are ineffective in controlling biofilm development. Recent studies demonstrate definite advantages in using natural compounds such as trans-cinnamaldehyde in thwarting biofilm assembly, and yet, the remarkable difficulty in delivering such hydrophobic bioactive molecules prevents further development. To address this critical challenge, we have developed an innovative platform composed of components with a proven track record of safety. We fabricated and thoroughly characterised porous silicon (pSi) microparticles to carry and deliver the natural phenyl propanoid trans-cinnamaldehyde (TC). We investigated its effects on preventing the development of cross-kingdom biofilms ( and ), typical of dental caries found in children. The prepared pSi microparticles were roughly cubic in structure with 70-75% porosity, to which the TC (pSi-TC) was loaded with about 45% efficiency. The pSi-TC particles exhibited a controlled release of the cargo over a 14-day period. Notably, pSi-TC significantly inhibited biofilms, specifically downregulating the glucan synthesis pathways, leading to reduced adhesion to the substrate. Acid production, a vital virulent trait for caries development, was also hindered by pSi-TC. This pioneering study highlights the potential to develop the novel pSi-TC as a dental caries-preventive material.
龋齿是一种可预防的疾病，由细菌和酵母组成的高度黏附、产酸生物膜引起。目前的龋齿预防方法在控制生物膜形成方面效果不佳。最近的研究表明，使用诸如反式肉桂醛等天然化合物在阻止生物膜组装方面具有明显优势，然而，递送此类疏水性生物活性分子存在的显著困难阻碍了其进一步发展。为应对这一关键挑战，我们开发了一个由具有可靠安全记录的成分组成的创新平台。我们制备并全面表征了用于携带和递送天然苯丙烷类反式肉桂醛（TC）的多孔硅（pSi）微粒。我们研究了其对预防儿童龋齿中典型的跨界生物膜（和）形成的影响。制备的pSi微粒结构大致呈立方体，孔隙率为70 - 75%，TC以约45%的效率负载于其上（pSi - TC）。pSi - TC微粒在14天内实现了所载物质的控释。值得注意的是，pSi - TC显著抑制生物膜，特别是下调葡聚糖合成途径，导致对底物的黏附减少。产酸是龋齿发展的一个重要致病特性，也受到pSi - TC的阻碍。这项开创性研究凸显了将新型pSi - TC开发为龋齿预防材料的潜力。
