Majewska Małgorzata, Kuźma Łukasz, Szymczyk Piotr
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego 1, 90-151 Lodz, Poland.
Plants (Basel). 2022 Jul 16;11(14):1861. doi: 10.3390/plants11141861.
synthesises tanshinones with multidirectional therapeutic effects. These compounds have a complex biosynthetic pathway, whose first rate limiting enzyme is 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR). In the present study, a new 1646 bp fragment of the gene consisting of a promoter, 5' untranslated region and part of a coding sequence was isolated and characterised in silico using bioinformatics tools. The results indicate the presence of a TATA box, tandem repeat and pyrimidine-rich sequence, and the absence of CpG islands. The sequence was rich in motifs recognised by specific transcription factors sensitive mainly to light, salicylic acid, bacterial infection and auxins; it also demonstrated many binding sites for microRNAs. Moreover, our results suggest that expression is possibly regulated during flowering, embryogenesis, organogenesis and the circadian rhythm. The obtained data were verified by comparison with microarray co-expression results obtained for . Alignment of the isolated sequence with other plant s indicated the presence of many common binding sites for transcription factors, including conserved ones. Our findings provide valuable information for understanding the mechanisms that direct transcription of the gene.
合成具有多向治疗作用的丹参酮。这些化合物具有复杂的生物合成途径，其首个限速酶是3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR）。在本研究中，分离出一个由启动子、5'非翻译区和部分编码序列组成的新的1646 bp基因片段，并使用生物信息学工具在计算机上进行了表征。结果表明存在TATA盒、串联重复序列和富含嘧啶的序列，且不存在CpG岛。该序列富含主要对光、水杨酸、细菌感染和生长素敏感的特定转录因子识别的基序；它还展示了许多微小RNA的结合位点。此外，我们的结果表明，在开花、胚胎发生、器官发生和昼夜节律过程中， 表达可能受到调控。通过与为 获得的微阵列共表达结果进行比较，验证了所得数据。将分离的 序列与其他植物 的序列进行比对，表明存在许多转录因子的共同结合位点，包括保守的结合位点。我们的研究结果为理解指导 基因转录的机制提供了有价值的信息。
