Chen Yue, Wang Yanting, Guo Juan, Yang Jian, Zhang Xiaodan, Wang Zixuan, Cheng Ying, Du Zewei, Qi Zhechen, Huang Yanbo, Dennis Mans, Wei Yukun, Yang Dongfeng, Huang Luqi, Liang Zongsuo
College of Life Sciences and Medicine, Key Laboratory of Plant Secondary Metabolism and Regulation in Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, China.
State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Front Plant Sci. 2022 Mar 3;12:820582. doi: 10.3389/fpls.2021.820582. eCollection 2021.
Tanshinones found in species are the main active compounds for the treatment of cardiovascular and cerebrovascular diseases, but their contents are hugely different in different species. For example, tanshinone IIA content in Diels f. Stib. is about 49 times higher than that in Bunge. The molecular mechanism responsible for this phenomenon remains largely unknown. To address this, we performed comparative transcriptomic and proteomic analyses of and . A total of 296 genes in and 125 genes in were highly expressed at both the transcriptional and proteome levels, including hormone signal regulation, fungus response genes, transcription factors, and CYP450. Among these differentially expressed genes, the expression of was particularly high in . Overexpression of in could significantly increase the content of tanshinone I and tanshinone IIA, which were 11.09 and 33.37 times of the control, respectively. Moreover, had a strong regulatory effect, elevating the expression levels of tanshinone pathway genes such as , and . For the WRKY family, 79 s were originally obtained and classified into three main groups. Collinearity analysis indicated a more specific extension of gene family in genus. In 55 species, only 37 species contained the sequence, and high expression in some L. species was often accompanied by high tanshinone accumulation. The above results suggest that is a highly effective regulator of tanshinone accumulation and may be a key factor resulting in high tanshinone accumulation in .
丹参中发现的丹参酮是治疗心脑血管疾病的主要活性成分，但不同物种中其含量差异巨大。例如，甘肃丹参中丹参酮IIA的含量比丹参高约49倍。造成这种现象的分子机制在很大程度上仍不清楚。为了解决这个问题，我们对甘肃丹参和丹参进行了比较转录组学和蛋白质组学分析。甘肃丹参中有296个基因和丹参中有125个基因在转录组和蛋白质组水平均高表达，包括激素信号调节、真菌反应基因、转录因子和CYP450。在这些差异表达基因中，[具体基因名称]在甘肃丹参中的表达尤其高。在丹参中过表达[具体基因名称]可显著增加丹参酮I和丹参酮IIA的含量，分别是对照的11.09倍和33.37倍。此外，[具体基因名称]具有很强的调控作用，可提高丹参酮途径基因如[具体基因名称]等的表达水平。对于WRKY家族，最初获得了79个序列并分为三个主要组。共线性分析表明[具体基因名称]基因家族在丹参属中有更特异的扩展。在55种丹参属植物中，只有37种含有[具体基因名称]序列，并且在一些丹参物种中高[具体基因名称]表达常伴随着高丹参酮积累。上述结果表明[具体基因名称]是丹参酮积累的高效调节因子，可能是导致甘肃丹参中高丹参酮积累的关键因素。
