Nayunigari Mithil Kumar, Suri Rominder, Andaluri Gangadhar
Department of Civil and Environmental Engineering, Temple University, 1947 North 12th St, Philadelphia, PA 19122, USA.
Polymers (Basel). 2022 Jul 8;14(14):2797. doi: 10.3390/polym14142797.
We report microbial resistance and catalytic activity of high viscosity cationic poly(proline-epichlorohydrin) composite (PRO-EPI) in the aqueous system. The PRO-EPI was prepared by a simple polycondensation, followed by FTIR, H NMR, SEM, DLS, viscosity, and DSC/TGA characterization. Several concentrations of the PRO-EPI were tested against Gram-negative ( and ) and Gram-positive () microorganisms. The antimicrobial screening revealed that PRO-EPI was a potent antimicrobial agent with the least inhibitory concentrations (MICs) of 128 µg/mL against Gram-negative microorganisms. The PRO-EPI indicated no inhibitory effect against Gram-positive microorganisms. It was determined that PRO-EPI contains polymeric-quaternary ammonium compounds that inactivate the Gram-negative microorganisms by a dual mode of action and carries domains for electrostatic interaction with the microbial membrane and an intracellular target. To study the removal of toxic industrial wastewater, congo red (CR) was tested using sodium borohydride as a reducing agent. Adsorption was achieved within 20 min at a rate constant of 0.92 ks. UV-vis spectra showed that the removal of CR in the reaction solution was due to the breakup of the azo (-N=N-) bonds and adsorption of aromatic fragments. PRO is biodegradable and non-toxic, and PRO-EPI was found to be both antimicrobial and also acts as a catalyst for the removal of congo red dye.
我们报道了高粘度阳离子聚（脯氨酸 - 环氧氯丙烷）复合材料（PRO - EPI）在水体系中的微生物抗性和催化活性。PRO - EPI通过简单的缩聚反应制备，随后进行了傅里叶变换红外光谱（FTIR）、氢核磁共振（H NMR）、扫描电子显微镜（SEM）、动态光散射（DLS）、粘度以及差示扫描量热法/热重分析法（DSC/TGA）表征。测试了几种浓度的PRO - EPI对革兰氏阴性菌（和）及革兰氏阳性菌（）的抗菌性能。抗菌筛选显示，PRO - EPI是一种强效抗菌剂，对革兰氏阴性微生物的最低抑菌浓度（MICs）为128 µg/mL。PRO - EPI对革兰氏阳性微生物无抑制作用。已确定PRO - EPI含有聚合季铵化合物，其通过双重作用模式使革兰氏阴性微生物失活，并带有与微生物膜和细胞内靶点进行静电相互作用的结构域。为研究有毒工业废水的去除，以硼氢化钠作为还原剂测试了刚果红（CR）。在20分钟内以0.92 ks的速率常数实现了吸附。紫外可见光谱表明，反应溶液中CR的去除是由于偶氮（-N=N-）键的断裂和芳香族片段的吸附。脯氨酸是可生物降解且无毒的，并且发现PRO - EPI既具有抗菌性，又可作为去除刚果红染料的催化剂。
