Department of Civil, Structural and Environmental Engineering, Trinity College Dublin, D02 PN40 Dublin, Ireland.
AMBER Research Centre and Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Trinity College Dublin, D02 PN40 Dublin, Ireland.
Sensors (Basel). 2022 Jul 17;22(14):5340. doi: 10.3390/s22145340.
The development of a reusable and low-cost urine glucose sensor can benefit the screening and control of diabetes mellitus. This study focused on the feasibility of employing microbial fuel cells (MFC) as a selective glucose sensor for continuous monitoring of glucose levels in human urine. Using MFC technology, a novel cylinder sensor (CS) was developed. It had a quick response time (100 s), a large detection range (0.3-5 mM), and excellent accuracy. More importantly, the CS could last for up to 5 months. The selectivity of the CS was validated by both synthetic and actual diabetes-negative urine samples. It was found that the CS's selectivity could be significantly enhanced by adjusting the concentration of the culture's organic matter. The CS results were comparable to those of a commercial glucose meter (recovery ranged from 93.6% to 127.9%) when the diabetes-positive urine samples were tested. Due to the multiple advantages of high stability, low cost, and high sensitivity over urine test strips, the CS provides a novel and reliable approach for continuous monitoring of urine glucose, which will benefit diabetes assessment and control.
开发一种可重复使用且低成本的尿糖传感器可以有利于糖尿病的筛查和控制。本研究专注于微生物燃料电池（MFC）作为一种选择性葡萄糖传感器用于连续监测人尿液中葡萄糖水平的可行性。使用 MFC 技术，开发了一种新型圆柱传感器（CS）。它具有快速的响应时间（100 s）、大的检测范围（0.3-5 mM）和优异的准确性。更重要的是，CS 可以持续使用长达 5 个月。通过合成和实际的非糖尿病尿液样本验证了 CS 的选择性。结果发现，通过调整培养物有机物的浓度可以显著提高 CS 的选择性。当测试阳性尿液样本时，CS 的结果与商业血糖仪（回收率范围为 93.6%至 127.9%）相当。由于 CS 在尿液检测条上具有高稳定性、低成本和高灵敏度的多重优势，为连续监测尿液葡萄糖提供了一种新颖而可靠的方法，将有利于糖尿病的评估和控制。
