Gomes Lucas Gabriel Rodrigues, Rodrigues Thaís Cristina Vilela, Jaiswal Arun Kumar, Santos Roselane Gonçalves, Kato Rodrigo Bentes, Barh Debmalya, Alzahrani Khalid J, Banjer Hamsa Jameel, Soares Siomar de Castro, Azevedo Vasco, Tiwari Sandeep
Laboratory of Cellular and Molecular Genetics (LGCM), PG Program in Bioinformatics, Department of Genetics, Ecology, and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, Brazil.
Centre for Genomics and Applied Gene Technology, Institute of Integrative Omics and Applied Biotechnology (IIOAB), Nonakuri, Purba Medinipur 721172, West Bengal, India.
Vaccines (Basel). 2022 Jun 25;10(7):1019. doi: 10.3390/vaccines10071019.
Syphilis, a sexually transmitted infection caused by the spirochete , has seen a resurgence over the past years. is capable of early dissemination and immune evasion, and the disease continues to be a global healthcare burden. The purpose of this study was to design a multi-epitope immunogen through an immunoinformatics-based approach. Multi-epitope immunogens constitute carefully selected epitopes belonging to conserved and essential bacterial proteins. Several physico-chemical characteristics, such as antigenicity, allergenicity, and stability, were determined. Further, molecular docking and dynamics simulations were performed, ensuring binding affinity and stability between the immunogen and TLR-2. An in silico cloning was performed using the pET-28a(+) vector and codon adaptation for . Finally, an in silico immune simulation was performed. The in silico predictions obtained in this work indicate that this construct would be capable of inducing the requisite immune response to elicit protection against . Through this methodology we have designed a promising potential vaccine candidate for syphilis, namely Tpme-VAC/LGCM-2022. However, it is necessary to validate these findings in in vitro and in vivo assays.
梅毒是一种由螺旋体引起的性传播感染疾病，在过去几年中出现了复发。它能够早期传播并逃避免疫，这种疾病仍然是全球医疗保健的负担。本研究的目的是通过基于免疫信息学的方法设计一种多表位免疫原。多表位免疫原由精心挑选的属于保守和必需细菌蛋白的表位组成。确定了几个物理化学特性，如抗原性、致敏性和稳定性。此外，进行了分子对接和动力学模拟，以确保免疫原与TLR-2之间的结合亲和力和稳定性。使用pET-28a(+)载体进行了电子克隆，并对[具体内容缺失]进行了密码子优化。最后，进行了电子免疫模拟。这项工作中获得的电子预测表明，该构建体能够诱导必要的免疫反应以引发针对[具体内容缺失]的保护。通过这种方法，我们设计了一种有前景的梅毒潜在疫苗候选物，即Tpme-VAC/LGCM-2022。然而，有必要在体外和体内试验中验证这些发现。
