Institute of Marine Biology, National Taiwan Ocean University, Keelung 20224, Taiwan.
Innocreate Bioscience Co., Ltd., Zhonghe District, New Taipei City 23557, Taiwan.
Viruses. 2022 Jun 21;14(7):1353. doi: 10.3390/v14071353.
Shrimp farming is an important economic activity. However, due to the spread of pathogens, shrimp aquaculture is becoming increasingly difficult. Many studies have confirmed that white spot syndrome virus (WSSV) recombinant proteins can inhibit viral infection. Among them, VP53 recombinant protein has been found to reduce mortality upon WSSV challenge. This study was conducted in Kaohsiung, Taiwan and reports the first field feeding trial to demonstrate that WSSV recombinant proteins can improve shrimp survival rates at a farming scale. Prior to the feeding trial, the shrimp were confirmed to be slightly infected with WSSV, strains causing acute hepatopancreatic necrosis disease (AHPND), non-AHPND strains, and (EHP), which are common pathogens that shrimp farmers often face. The shrimp were then divided into two groups: a control group (C group) fed with a commercial diet and a protein group (P group) fed with the same commercial feed with VP53 recombinant protein. Our findings indicated that the survival rate and expression of immune genes of the P group were higher than those of the C group. The intestinal microbiota of the two groups were also analysed. Collectively, our results confirmed that the recombinant WSSV envelope protein derivative can be used as an effective anti-virus biological agent in shrimp farms.
虾类养殖是一项重要的经济活动。然而，由于病原体的传播，虾类养殖业越来越困难。许多研究证实，白斑综合征病毒（WSSV）重组蛋白可以抑制病毒感染。其中，VP53 重组蛋白已被发现可降低 WSSV 攻毒后的死亡率。本研究在台湾高雄进行，报告了首例田间饲养试验，证明 WSSV 重组蛋白可提高养殖规模下虾的存活率。在饲养试验之前，已确认虾类受到 WSSV 轻度感染，感染急性肝胰腺坏死病（AHPND）的毒株、非 AHPND 株和弧菌（EHP），这些都是虾农经常面临的常见病原体。然后将虾分为两组：对照组（C 组）喂食商业饲料，蛋白组（P 组）喂食相同的商业饲料，添加 VP53 重组蛋白。我们的研究结果表明，P 组的存活率和免疫基因表达均高于 C 组。还对两组的肠道微生物群进行了分析。总的来说，我们的结果证实了重组 WSSV 囊膜蛋白衍生物可用作虾场的有效抗病毒生物制剂。
