Institutes of Biomedical Sciences, Shanxi University, 92 Wucheng Road, Taiyuan 030006, China.
The Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Shanxi University, 92 Wucheng Road, Taiyuan 030006, China.
Viruses. 2022 Jul 2;14(7):1467. doi: 10.3390/v14071467.
Discrepancies in human hepatitis delta virus (HDV) genotypes impact the virus' biological behavior, clinical manifestation, and treatment response. Herein, this report aims to explore the role of recombination in the worldwide genotypic distribution and genetic diversity of HDV. Three-hundred-forty-eight human HDV full-length genomic sequences of ~1678 nt in length, isolated in twenty-eight countries worldwide between 1986 and 2018, were analysed. Similarity analysis and recombination mapping were performed, and forty-eight recombination events were identified, twenty-nine of which were isolated from Kyrgyzstan and determined to be involved in the diversity and extension of HDV sub-genotypes. HDV recombination occurred only between the genetically close genotypes (genotype 5 and genotype 2) or mainly within genotype 1, suggesting the complex replicative molecular mechanisms of HDV-RNA. The global distribution and classification of HDV genotypes have been updated, indicating that HDV recombination is one of the driving forces behind the biodiversity and the evolution of human HDV genomes. The outcome analysis suggests that the expansion of HDV sub-genotypes and the complex recombination networks might be related to the genomic character of Kyrgyzstan circulating strains and extensive mobility within countries and across borders. These findings will be of great importance in formulating more effective public health HDV surveillance strategies and guiding future molecular and epidemiological research to achieve better clinical outcomes.
人类乙型肝炎 delta 病毒（HDV）基因型的差异影响病毒的生物学行为、临床表现和治疗反应。本报告旨在探讨重组在全球 HDV 基因型分布和遗传多样性中的作用。分析了来自全球 28 个国家的 348 个人类 HDV 全长基因组序列，长度约为 1678 个核苷酸。进行了相似性分析和重组图谱绘制，鉴定出 48 个重组事件，其中 29 个来自吉尔吉斯斯坦，涉及 HDV 亚基因型的多样性和扩展。HDV 重组仅发生在遗传上接近的基因型（基因型 5 和基因型 2）之间，或主要发生在基因型 1 内，表明 HDV-RNA 的复杂复制分子机制。更新了全球 HDV 基因型的分布和分类，表明 HDV 重组是人类 HDV 基因组多样性和进化的驱动力之一。结果分析表明，HDV 亚基因型的扩展和复杂的重组网络可能与吉尔吉斯斯坦流行株的基因组特征以及国内和跨境的广泛流动性有关。这些发现对于制定更有效的公共卫生 HDV 监测策略以及指导未来的分子和流行病学研究以实现更好的临床结果具有重要意义。
