Suppr超能文献

从伊拉克摩苏尔当地菌株中分离与鉴定,并研究磷酸钾对其生长的影响。

Isolation and Identification of from the Local Strain in Mosul, Iraq, and Investigation of Potassium Phosphate Effect on its Growth.

作者信息

Yahya Allawi M, Salim Al-Taie B, Hmoshi R M

机构信息

Environmental Science Department, College of Environmental Science and Technologies, University of Mosul, Mosul, Iraq.

出版信息

Arch Razi Inst. 2022 Feb 28;77(1):421-427. doi: 10.22092/ARI.2021.356684.1896. eCollection 2022 Feb.

Abstract

species is one of the most common microscopic filamentous fungi that have been isolated from widespread substrates. In this study, soil samples from different areas of Mosul, Iraq, were examined by morphological and molecular methods. Fungi were isolated and grown in diagnostic culture media, including Czapek Yeast Extract Agar, Malt Extract Agar, and 25% Glycerol nitrate agar at different temperatures of 15, 20, 25, and 30°C. The growth rate of the fungus was also evaluated in the culture containing KHPO. In the molecular method, the obtained fragment was sequenced using the Internal Transcribed Spacer region primers after replication. Morphological evaluation of both macroscopic and microscopic features revealed that strains were , and molecular methods used have confirmed the isolated strain. The results of this study showed a decrease in the diameters of the colonies at different growth temperatures when doubling the amount of KHPO. In addition, the findings revealed that compared to growth inhibition in the control groups, the maximum growth inhibition was 16 and 29 mm on Czapek Dox Agar (modified) medium at incubation temperatures of 20°C and 25°C, respectively. As a result, KHPO has an important role in inhibiting growth; therefore, it can be used as Fungicide.

摘要

该物种是从广泛的基质中分离出来的最常见的微观丝状真菌之一。在本研究中,采用形态学和分子方法对伊拉克摩苏尔不同地区的土壤样本进行了检测。真菌在诊断培养基中分离培养,包括察氏酵母提取物琼脂、麦芽提取物琼脂和25%甘油硝酸盐琼脂,培养温度分别为15、20、25和30°C。还在含有KHPO的培养基中评估了真菌的生长速率。在分子方法中,复制后使用内转录间隔区引物对获得的片段进行测序。对宏观和微观特征的形态学评估表明,菌株为 ,所使用的分子方法已证实了分离出的菌株。本研究结果表明,当KHPO量加倍时,不同生长温度下菌落直径减小。此外,研究结果显示,与对照组的生长抑制相比,在20°C和25°C的培养温度下,在改良察氏培养基上的最大生长抑制分别为16和29毫米。因此,KHPO在抑制生长方面具有重要作用;因此,它可以用作杀菌剂。

相似文献

本文引用的文献

1
The phosphate language of fungi.真菌的磷酸盐语言。
Trends Microbiol. 2022 Apr;30(4):338-349. doi: 10.1016/j.tim.2021.08.002. Epub 2021 Aug 31.
8
Molecular diagnostics in medical mycology.医学真菌学中的分子诊断。
Nat Commun. 2018 Dec 3;9(1):5135. doi: 10.1038/s41467-018-07556-5.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验