蝎毒致死性和细胞毒性评价
Evaluation of Lethality and Cytotoxic Effect of Scorpion Venom.
机构信息
Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Department of Biology, Tofigh Daru Research and Engineering Company, Tehran, Iran.
出版信息
Arch Razi Inst. 2022 Feb 28;77(1):29-36. doi: 10.22092/ARI.2021.353302.1595. eCollection 2022 Feb.
The results of numerous studies have revealed that some deadly scorpion venoms are composed of various bioactive molecules that have significant cytotoxic effects on cancer cells. In this study, the lethality and cytotoxic effect of venom were evaluated in different cancer cell lines. Through MTT assay, the cytotoxic effects of scorpion venom were analyzed on the MCF-7, A549, AGS, HepG2, and Ht-29 cancer cell lines and Hu02 normal cells. To this end, six venom fractions were obtained through a Sephadex G-50 column, and the cytotoxic effects of isolated fractions were evaluated on A549 lung cancer cells. The median lethal dose of scorpion venom was determined at 0.73 mg/kg by intravenous administration of different venom doses in male BALB/c mice according to the Spearman-Karber method. The scorpion whole venom had a significant cytotoxic effect on MCF-7, A549, and AGS cells. The treatment of A549 cells with various concentrations of fraction F1 showed that this fraction significantly induced growth inhibitory effect on the cells in a dose-dependent manner, compared to untreated cells.
大量研究结果表明，某些致命的蝎子毒液由各种具有显著细胞毒性的生物活性分子组成，对癌细胞有显著的细胞毒性作用。在本研究中，评估了毒液在不同癌细胞系中的致死性和细胞毒性作用。通过 MTT assay 分析了 scorpion 毒液对 MCF-7、A549、AGS、HepG2 和 Ht-29 癌细胞系和 Hu02 正常细胞的细胞毒性作用。为此，通过 Sephadex G-50 柱获得了六个毒液级分，并评估了分离级分对 A549 肺癌细胞的细胞毒性作用。根据 Spearman-Karber 法，通过静脉给予不同剂量的毒液，确定 scorpion 毒液的半数致死剂量为 0.73mg/kg。 scorpion 全毒液对 MCF-7、A549 和 AGS 细胞具有显著的细胞毒性作用。用不同浓度的 F1 级分处理 A549 细胞，与未处理的细胞相比，该级分显著地以剂量依赖性方式诱导细胞生长抑制作用。
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