Mount Lebanon Hospital, Azoury IVF Clinic, Beirut, Lebanon.
Faculty of Medicine, Regenerative Medicine and Inflammation Laboratory, Saint-Joseph University, Beirut, Lebanon.
Andrology. 2020 Jan;8(1):191-200. doi: 10.1111/andr.12648. Epub 2019 May 12.
Among new therapies emerging in the medical field, the use of platelet-rich plasma (PRP) in human reproduction has not yet been explored.
This study aimed at investigating the effect of autologous PRP on sperm parameters in the presence and absence of H O .
Semen samples were collected from 30 healthy men in their fourth decade attending Azoury IVF clinic. Spermatozoa, cultured in the presence or absence of 10 μM H O , were left untreated or treated with increasing concentrations of PRP (2, 5, and 10%). After 24-h incubation, ROS levels were assessed and sperm parameters were evaluated.
Our results highlight the harmful effect of H O on sperm parameters, showing an increase in the percentage of reactive oxygen species (ROS)-positive cells, vacuolization, and sperm DNA fragmentation, and a decrease in progressive and total motility in the H O -treated group compared to non-stressed spermatozoa. When samples were treated with PRP, an improvement of the studied parameters was noted mainly with 2% PRP, thus regarded as the best concentration to achieve a positive effect on sperm parameters. Indeed, non-stressed and stressed spermatozoa treated with 2% PRP showed a significant increase in progressive and total motility, coupled with a decrease in ROS-positive cells, DNA fragmentation, vacuolization, and dead cells compared to the untreated group. In contrast, no significant difference in cell morphology was found between the two groups. Moreover, 2% PRP treatment enhanced sperm parameters and prevented cell death in H O -exposed spermatozoa as compared to freshly collected semen.
We suggest that PRP because of its wide arrays of growth factors included in his alpha granules contributes to the inhibition of ROS through the antioxidant, anti-apoptotic activity.
Autologous PRP improves the quality of the sperm, more so in the presence of an H O -induced OS.
在医学领域涌现的新疗法中，富血小板血浆（PRP）在人类生殖中的应用尚未得到探索。
本研究旨在调查在存在和不存在 H O 的情况下，自体 PRP 对精子参数的影响。
从 30 名 40 岁出头的在 Azoury IVF 诊所就诊的健康男性中收集精液样本。将精子在存在或不存在 10 μM H O 的情况下培养，然后不进行处理或用不同浓度的 PRP（2、5 和 10%）进行处理。孵育 24 小时后，评估 ROS 水平并评估精子参数。
我们的结果突出了 H O 对精子参数的有害影响，显示 ROS 阳性细胞的百分比增加、空泡化和精子 DNA 碎片化增加，以及在 H O 处理组中与非应激精子相比，前向运动和总运动能力下降。当用 PRP 处理样本时，观察到所研究的参数得到改善，主要是用 2%的 PRP，因此被认为是获得对精子参数的积极影响的最佳浓度。事实上，用 2%的 PRP 处理的非应激和应激精子显示出前向运动和总运动能力显著增加，同时 ROS 阳性细胞、DNA 碎片化、空泡化和死细胞的数量减少，与未处理组相比。相比之下，两组之间的细胞形态没有发现显著差异。此外，与新鲜采集的精液相比，2%的 PRP 处理可增强 H O 暴露的精子的精子参数并防止细胞死亡。
我们认为 PRP 由于其α颗粒中包含的广泛的生长因子，通过抗氧化、抗凋亡活性有助于抑制 ROS。
自体 PRP 可提高精子质量，在存在 H O 诱导的 OS 时更为明显。
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