Department of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, 00790 Helsinki, Finland.
Centre for Structural and Functional Genomics, Concordia University, Montréal, QC H4B 1R6, Canada.
Biomolecules. 2022 Jul 22;12(8):1017. doi: 10.3390/biom12081017.
The unique ability of basidiomycete white rot fungi to degrade all components of plant cell walls makes them indispensable organisms in the global carbon cycle. In this study, we analyzed the proteomes of two closely related white rot fungi, and , during eight-week cultivation on solid spruce wood. Plant cell wall degrading carbohydrate-active enzymes (CAZymes) represented approximately 5% of the total proteins in both species. A core set of orthologous plant cell wall degrading CAZymes was shared between these species on spruce suggesting a conserved plant biomass degradation approach in this clade of basidiomycete fungi. However, differences in time-dependent production of plant cell wall degrading enzymes may be due to differences among initial growth rates of these species on solid spruce wood. The obtained results provide insight into specific enzymes and enzyme sets that are produced during the degradation of solid spruce wood in these fungi. These findings expand the knowledge on enzyme production in nature-mimicking conditions and may contribute to the exploitation of white rot fungi and their enzymes for biotechnological applications.
担子菌纲白腐真菌独特的降解植物细胞壁所有成分的能力，使它们成为全球碳循环中不可或缺的生物。在这项研究中，我们分析了两种密切相关的白腐真菌（ 和 ）在固体云杉上培养 8 周时的蛋白质组。在这两个物种中，植物细胞壁降解碳水化合物活性酶（CAZymes）约占总蛋白的 5%。在云杉上，这些物种之间共享了一组核心的同源植物细胞壁降解 CAZymes，这表明在担子菌真菌的这个分支中存在保守的植物生物质降解方法。然而，植物细胞壁降解酶的时间依赖性产生的差异可能是由于这些物种在固体云杉上初始生长速度的不同造成的。研究结果为这些真菌在降解固体云杉过程中产生的特定酶和酶组提供了深入的了解。这些发现扩展了在模拟自然条件下酶产生的知识，并可能有助于白腐真菌及其酶在生物技术应用中的开发。
