School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong, 510640, China.
Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, Guangdong, 510610, China.
Carbohydr Polym. 2020 Feb 15;230:115647. doi: 10.1016/j.carbpol.2019.115647. Epub 2019 Nov 19.
The depolymerization effect of UV/HO on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied. The structural changes of PSF before and after UV/HO treatment were analyzed, and molecular weight changes during in vitro digestion were determined. Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L HO, and the depolymerization effect of UV/HO was significantly higher than that of UV or HO alone. In addition, the UV/HO treatment had a high recovery rate of total sugar (93.54 %) and clearance rate of protein (76.34 %). The monosaccharide composition showed that UV/HO treatment could increase the mole percentage of mannose (37.44 %) and decrease the mole percentage of fucose (14.88 %). The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/HO treatment depolymerized PSF. Rheological studies indicated that PSF with UV/HO treatment had lower viscosity. In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/HO treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated intestinal juice. This studied suggested that the preparation and application of functional PSF with low molecular weight might be beneficial.
研究了 UV/HO 对马尾藻多糖（PSF）的解聚作用，PSF 是一种褐藻。分析了 UV/HO 处理前后 PSF 的结构变化，并测定了体外消化过程中分子量的变化。结果表明，在 2 h 内，UV/150 mmol/L HO 使 PSF 的分子量从约 289 kDa 降低至约 12.6 kDa，UV/HO 的解聚效果明显高于单独的 UV 或 HO。此外，UV/HO 处理具有较高的总糖回收率（93.54%）和蛋白质清除率（76.34%）。单糖组成表明，UV/HO 处理可以增加甘露糖（37.44%）的摩尔百分比，降低岩藻糖（14.88%）的摩尔百分比。螺旋-卷曲转变、X 射线衍射（XRD）和原子力显微镜（AFM）成像表明，UV/HO 处理使 PSF 解聚。流变学研究表明，经 UV/HO 处理的 PSF 具有较低的粘度。体外消化表明，经体外胃肠道模拟消化后，PSF 几乎未被消化，但在模拟胃液的低酸环境中，经 UV/HO 处理的 PSF 可以被消化，但在模拟肠液中几乎未被消化。本研究表明，制备和应用低分子量的功能性 PSF 可能是有益的。
