Roslan Muhamad Aidilfitri Mohamad, Sobri Zulfazli M, Zuan Ali Tan Kee, Abdul Rahman Nor Aini
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Biology (Basel). 2022 Jul 25;11(8):1107. doi: 10.3390/biology11081107.
There is limited evidence that Enterobacter hormaechei can improve plant physiology and yield through soil phosphate (P) and potassium (K) amelioration. This study unraveled the effect of different soil inoculation methods i.e., free-cell and encapsulated (alginate bead containing sugar-protein hydrolysate and molasses) E. hormaechei 40a with different rates of PK-fertilization on okra P and K uptake, and soil rhizosphere bacterial community. The results revealed that 3HB (half-dose PK-fertilizer + encapsulated strain 40a) had the highest soil available P (SAP) and K (SAK), as well as P and K uptake for all plant organs, followed by 3F (full-dose PK-fertilizer), 3HI (half-dose PK-fertilizer + free-cell strain 40a), and 3H (half-dose PK-fertilizer), and improved yield by up to 75.6%. Both inoculated and full-dose fertilizer treatments produced larger pods (>15 cm) compared to 3H. We discovered increased bacterial richness and diversity in both 3HB and 3HI samples compared to uninoculated treatments. Both 3HB and 3F treatments were positively correlated with the increasing abundance of Acidobacteriales, Burkholderia caballeronia paraburkholderia, Gemmataceae, and Sphingomonas along with the SAP and SAK. The plant-beneficial effect of one-time 3HB treatment on okra growth and yield was comparable to biweekly inoculation in 3HI, suggesting a new cost-effective farming approach in precision agriculture.
关于霍氏肠杆菌可通过改善土壤磷（P）和钾（K）来提高植物生理机能和产量的证据有限。本研究揭示了不同土壤接种方法，即游离细胞和包囊化（含糖蛋白水解物和糖蜜的藻酸盐珠粒）的霍氏肠杆菌40a，在不同磷钾肥施用量下对秋葵磷钾吸收及土壤根际细菌群落的影响。结果显示，3HB（半剂量磷钾肥+包囊化菌株40a）的土壤有效磷（SAP）和有效钾（SAK）含量最高，所有植物器官的磷钾吸收量也最高，其次是3F（全剂量磷钾肥）、3HI（半剂量磷钾肥+游离细胞菌株40a）和3H（半剂量磷钾肥），产量提高了75.6%。与3H相比，接种处理和全剂量肥料处理均产生了更大的豆荚（>15厘米）。我们发现，与未接种处理相比，3HB和3HI样本中的细菌丰富度和多样性均有所增加。3HB和3F处理均与酸杆菌目、类伯克霍尔德菌属、芽单胞菌科和鞘氨醇单胞菌属丰度的增加以及土壤有效磷和有效钾呈正相关。一次性3HB处理对秋葵生长和产量的有益效果与3HI中的双周接种相当，这表明了精准农业中一种新的经济高效的种植方法。
