State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, 666 Wusu Street, Hangzhou 311300, China.
Key Laboratory of Modern Silvicultural Technology of Zhejiang Province, Hangzhou 311300, China.
Genes (Basel). 2022 Jul 22;13(8):1305. doi: 10.3390/genes13081305.
Protein kinases play an essential role in plants' responses to environmental stress signals. (sucrose non-fermenting 1-related protein kinase 2) is a plant-specific protein kinase that plays a crucial role in abscisic acid and abiotic stress responses in some model plant species. In apple, corn, rice, pepper, grapevine, , potato, and tomato, a genome-wide study of the SnRK2 protein family was performed earlier. The genome-wide comprehensive investigation was first revealed to categorize the genes in the . The five genes found in the genome were highlighted in this study. The structural gene variants, 3D structure, chromosomal distributions, motif analysis, phylogeny, subcellular localization, cis-regulatory elements, expression profiles in dormant buds, and photoperiod and chilling responses were all investigated in this research. The five genes from were grouped into groups (I-IV) based on phylogeny analysis, with three being closely related to other species. Five hormones-, six stress-, two growths and biological process-, and two metabolic-related responsive elements were discovered by studying the cis-elements in the promoters. According to the expression analyses, all five genes were up- and down-regulated in response to abscisic acid (ABA), photoperiod, chilling, and chilling, as well as photoperiod treatments. Our findings gave insight into the family genes in and opened up new study options.
蛋白激酶在植物对环境胁迫信号的响应中发挥着重要作用。(蔗糖非发酵 1 相关蛋白激酶 2)是一种植物特异性蛋白激酶,在一些模式植物物种的脱落酸和非生物胁迫响应中起着关键作用。在苹果、玉米、水稻、辣椒、葡萄、土豆和番茄中,先前对 SnRK2 蛋白家族进行了全基因组研究。本研究首次全面揭示了该家族的分类。本研究重点研究了 基因组中发现的 5 个 基因。该研究还对结构基因变异体、3D 结构、染色体分布、基序分析、系统发育、亚细胞定位、顺式调控元件、休眠芽中的表达谱以及光周期和冷胁迫响应进行了研究。根据系统发育分析,将 中的 5 个 基因分为 I-IV 组,其中 3 个与其他物种密切相关。通过研究启动子中的顺式元件,发现了 5 个激素、6 个应激、2 个生长和生物过程以及 2 个代谢相关的响应元件。根据表达分析,所有 5 个基因都对脱落酸(ABA)、光周期、冷胁迫和冷胁迫以及光周期处理有上调和下调的反应。我们的研究结果深入了解了 中的 家族基因,并为新的研究提供了选择。