Taylor R G, Pérez T R
Arch Invest Med (Mex). 1978;9 Suppl 1:363-6.
A new fluorescent immunoassay system (FIAX TM) has permitted development of a promising serologic test for amebiasis. The test is similar but far superior to the soluble antigen fluorescent antibody test. The main differences are the unique StiQ TM sampler and the flourometer itself. Quantitative results can be obtained from a single standard dilution of serum. The procedure is simple to perform, gives objective readings, and uses the proven principle of immunofluorescence. It is possible to test for different immunoglobulin classes and to use purified antigen prepared from axenically cultured Entamoeba histolytica. Conservation of reagents is comparable to microplate systems, and results are available in less than two hours. FIAX TM for amebiasis has demonstrated excellent discriminating power between amebic and nonamebic sera. Sensitivity is 100 percent for extra-intestinal amebiasis (33 sera), 90 percent for symptomatic intestinal amebiasis (20 sera), and 43 percent for asymptomatic amebiasis (seven sera). Specificity is 97 percent (158 sera).
一种新型荧光免疫分析系统（FIAX TM）已使得开发出一种有前景的阿米巴病血清学检测方法成为可能。该检测方法与可溶性抗原荧光抗体检测相似，但远优于后者。主要区别在于独特的StiQ TM采样器和荧光计本身。通过血清的单一标准稀释即可获得定量结果。该检测程序操作简单，读数客观，并采用了经过验证的免疫荧光原理。可以检测不同的免疫球蛋白类别，并使用从无共生培养的溶组织内阿米巴制备的纯化抗原。试剂的保存情况与微孔板系统相当，不到两小时即可获得结果。用于阿米巴病的FIAX TM在区分阿米巴血清和非阿米巴血清方面显示出出色的鉴别能力。肠外阿米巴病（33份血清）的敏感性为100%，有症状的肠道阿米巴病（20份血清）的敏感性为90%，无症状阿米巴病（7份血清）的敏感性为43%。特异性为97%（158份血清）。
