Sidorova Daria E, Skripka Mariia I, Khmel Inessa A, Koksharova Olga A, Plyuta Vladimir A
Institute of Molecular Genetics of National Research Center "Kurchatov Institute", Kurchatov Sq. 2, 123182 Moscow, Russia.
Department of Biotechnology, Mendeleev University of Chemical Technology of Russia, 125480 Moscow, Russia.
Microorganisms. 2022 Jul 26;10(8):1512. doi: 10.3390/microorganisms10081512.
Volatile organic compounds (VOCs) emitted by bacteria play an important role in the interaction between microorganisms and other organisms. They can inhibit the growth of phytopathogenic microorganisms, modulate plant growth, and serve as infochemicals. Here, we investigated the effects of ketones, alcohols, and terpenes on the colony biofilms of plant pathogenic strains and swimming motility, which can play an important role in the formation of biofilms. It was shown that 2-octanone had the greatest inhibitory effect on biofilm formation, acting in a small amount (38.7 g/m). Ketone 2-butanone and unsaturated ketone β-ionone reduced the formation of biofilms at higher doses (145.2-580.6 and 387.1-1548.3 g/m, respectively, up to 2.5-5 times). Isoamyl alcohol and 2-phenylethanol decreased the formation of biofilms at doses of 88.7 and 122.9 g/m by 1.7 and 5 times, respectively, with an increased effect at 177.4 and 245.9 g/m, respectively. The agrobacteria cells in mature biofilms were more resistant to the action of ketones and alcohols. These VOCs also suppressed the swimming motility of agrobacteria; the radius of swimming zones decreased ~from 2 to 5 times. Terpenes (-)-limonene and (+)-α-pinene had no significant influence on the colony biofilms and swimming motility at the doses used. The results obtained represent new information about the effect of VOCs on biofilms and the motility of bacteria.
细菌释放的挥发性有机化合物（VOCs）在微生物与其他生物体之间的相互作用中发挥着重要作用。它们可以抑制植物病原微生物的生长、调节植物生长，并作为信息化合物。在此，我们研究了酮类、醇类和萜类化合物对植物病原菌菌株菌落生物膜和游泳运动性的影响，而游泳运动性在生物膜形成中可发挥重要作用。结果表明，2-辛酮对生物膜形成的抑制作用最大，少量（38.7 g/m）即可起作用。酮类2-丁酮和不饱和酮β-紫罗兰酮在较高剂量（分别为145.2 - 580.6 g/m和387.1 - 1548.3 g/m，高达2.5 - 5倍）时可减少生物膜的形成。异戊醇和2-苯乙醇在剂量为88.7和122.9 g/m时，分别使生物膜形成减少1.7倍和5倍，在剂量分别为177.4和245.9 g/m时作用增强。成熟生物膜中的农杆菌细胞对酮类和醇类的作用更具抗性。这些VOCs还抑制了农杆菌的游泳运动性；游泳区域半径减小了约2至5倍。在所使用的剂量下，萜类化合物（-）-柠檬烯和（+）-α-蒎烯对菌落生物膜和游泳运动性没有显著影响。所获得的结果代表了关于VOCs对生物膜和细菌运动性影响的新信息。
