从污水样本中分离和鉴定裂解噬菌体 vB_VpaP_GHSM17

Isolation and Characterization of a Lytic Phage vB_VpaP_GHSM17 from Sewage Samples.

机构信息

School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.

Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology, Guangzhou 510006, China.

出版信息

Viruses. 2022 Jul 22;14(8):1601. doi: 10.3390/v14081601.

is a major foodborne pathogen and the main cause of diarrheal diseases transmitted by seafood such as fish, shrimp, and shellfish. In the current study, a novel lytic phage infecting , vB_VpaP_GHSM17, was isolated from the sewage of a seafood market, Huangsha, Guangzhou, and its morphology, biochemistry, and taxonomy features were identified. Morphological observation revealed that GHSM17 had an icosahedral head with a short, non-contractile tail. The double-stranded DNA genome of GHSM17 consisted of 43,228 bp with a GC content of 49.42%. In total, 45 putative ORFs were identified in the GHSM17 genome. Taxonomic analysis indicated GHSM17 belonging to genus , family In addition, GHSM17 was stable over a wide range of temperatures (20-60 °C) and pH (5-11) and was completely inactivated after 70 min of ultraviolet irradiation. The bacterial inhibition assay revealed that GHSM17 could inhibit the growth of within 8 h. The results support that phage GHSM17 may be a potential candidate in the biological control of contamination in aquaculture.

是一种主要的食源性致病菌，也是鱼类、虾类和贝类等海鲜传播的腹泻病的主要原因。在目前的研究中，从广州黄沙海鲜市场的污水中分离到一种感染的新型裂解噬菌体，命名为 vB_VpaP_GHSM17，并对其形态、生化和分类特征进行了鉴定。形态观察表明，GHSM17 具有一个二十面体头部和一个短的、不可收缩的尾部。GHSM17 的双链 DNA 基因组由 43,228 bp 组成，GC 含量为 49.42%。在 GHSM17 基因组中总共鉴定出 45 个推定的 ORF。分类分析表明 GHSM17 属于属，科此外，GHSM17 在较宽的温度（20-60°C）和 pH（5-11）范围内稳定，经过 70 分钟紫外线照射后完全失活。细菌抑制试验表明，GHSM17 可在 8 小时内抑制的生长。结果表明，噬菌体 GHSM17 可能是水产养殖中控制污染的潜在候选生物。