新型噬菌体 vB_VpaP_DE10 的特性分析与基因组学研究

Characterization and Genomic Analysis of Novel Phage vB_VpaP_DE10.

机构信息

College of Agriculture, College of Food Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

出版信息

Viruses. 2022 Jul 23;14(8):1609. doi: 10.3390/v14081609.

In the present study, a novel lytic phage, vB_VpaP_DE10, was isolated from sewage samples collected in Guangzhou city, China. Transmission electron microscopy revealed that phage vB_VpaP_DE10 has an icosahedral head (52.4 ± 2.5 nm) and a short non-contracted tail (21.9 ± 1.0 nm). Phage vB_VpaP_DE10 lysed approximately 31% (8/26) of the antibiotic-resistant strains tested. A one-step growth curve showed that phage vB_VpaP_DE10 has a relatively long latency time of 25 min and a burst size of ~19 PFU per cell. The genome of phage vB_VpaP_DE10 is a 42,871-bp-long dsDNA molecule with a G + C content of 49.19% and is predicted to contain 46 open reading frames, 26 of which are predicted to be related to functions such as phage structure, packaging, host lysis, and DNA metabolism. Sequence comparisons suggested that vB_VpaP_DE10 is a member of the genus within the family . Morphological and genomic analysis indicated that vB_VpaP_DE10 is a novel phage.

在本研究中，从中国广州市采集的污水样本中分离到一种新型裂解噬菌体 vB_VpaP_DE10。透射电子显微镜显示噬菌体 vB_VpaP_DE10 具有二十面体头部（52.4 ± 2.5nm）和短非收缩尾部（21.9 ± 1.0nm）。噬菌体 vB_VpaP_DE10 裂解了约 31%（8/26）测试的抗生素抗性菌株。一步生长曲线表明，噬菌体 vB_VpaP_DE10 的潜伏期相对较长，为 25 分钟，爆发量约为每个细胞 19 个 PFU。噬菌体 vB_VpaP_DE10 的基因组是一个 42871bp 的 dsDNA 分子，GC 含量为 49.19%，预测包含 46 个开放阅读框，其中 26 个预测与噬菌体结构、包装、宿主裂解和 DNA 代谢等功能有关。序列比较表明，vB_VpaP_DE10 是科中的一个属。形态和基因组分析表明，vB_VpaP_DE10 是一种新型噬菌体。