Hsu Te-Ken, Chen Yi-Yin, Li Shiao-Wen, Shih Hui-Yu, Chou Hsin-Yiu, Hsu Jeff Chia-Kai, Wang Han-Ching, Chen Li-Li
Institute of Marine Biology, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung, 20224, Taiwan, ROC.
Innocreate Bioscience Co., Ltd, New Taipei City, Taiwan, ROC.
Sci Rep. 2025 Jan 22;15(1):2801. doi: 10.1038/s41598-025-85513-1.
Vibrio parahaemolyticus is pathogenic to both humans and marine animals. Antimicrobial-resistant (AMR) bacteria have been reported to cause mortalities in shrimp, with phage therapy presenting an alternative and eco-friendly biocontrol strategy for controlling bacterial diseases. Therefore, this study aimed to isolate and characterize phages for their applicability in lysing Vibrio parahaemolyticus. A novel phage vB_VpaS_BP15 (BP15) belonged to the subfamily Queuovirinae with an icosahedral head measuring 69.11 ± 5.38 nm in length and 65.40 ± 6.89 nm in width, and a non-contractile sheathed tail measuring 139.81 ± 14.79 nm. The one-step growth curve indicated a latent period of 30 min and a burst size of 120 PFUs per cell. Phage BP15 exhibited tolerance to a range of temperatures and pH values. Infection dynamic curves demonstrated that BP15 was highly effective against BCRC12959 at MOIs ranging from 0.01 to 10; even at a low multiplicity of infection (MOI) of 0.001, BP15 still caused growth retention. Phage BP15 possessed a circular double-stranded DNA of 59,584 bp with a G + C content of 46.7% and lacked tRNA genes, virulence genes, and lysogeny genes. These findings highlight the promising potential of phage BP15 as a biocontrol agent against Vibrio parahaemolyticus in Taiwan.
副溶血性弧菌对人类和海洋动物均具有致病性。据报道，耐药菌会导致虾类死亡，而噬菌体疗法是一种控制细菌性疾病的替代且环保的生物防治策略。因此，本研究旨在分离和鉴定噬菌体，以评估其裂解副溶血性弧菌的适用性。一种新型噬菌体vB_VpaS_BP15（BP15）属于Queuovirinae亚科，其二十面体头部长度为69.11±5.38nm，宽度为65.40±6.89nm，非收缩性带鞘尾部长度为139.81±14.79nm。一步生长曲线表明潜伏期为30分钟，每个细胞的裂解量为120个噬菌斑形成单位。噬菌体BP15在一系列温度和pH值条件下均具有耐受性。感染动态曲线表明，BP15在感染复数（MOI）为0.01至10的范围内对BCRC12959具有高效性；即使在低感染复数（MOI）为0.001时，BP15仍能导致生长抑制。噬菌体BP15拥有一条59,584bp的环状双链DNA，G+C含量为46.7%，且缺乏tRNA基因、毒力基因和溶原性基因。这些发现突出了噬菌体BP15作为台湾地区防治副溶血性弧菌生物防治剂的潜在应用前景。
